口腔微生物生态英文版指导.pptVIP

Oral Microbial Ecology DENT 5301 Introduction to Oral Biology Dr. Joel Rudney Oral microbial diversity The “we know what we can grow” bias Emphasis on species recovered from culture Revolutionized by molecular methods for species ID Species signatures in 16S ribosomal RNA sequences Does not require microbial culture Perhaps 700+ distinct oral species Most have never been cultured Gram+, Gram-, cocci, rods, filaments, spirochetes, etc. Include some very exotic taxonomic groups (Archea) Termite guts and other extreme environments Fungi and viruses are all in the mix Biofilm on tooth surfaces Adherence and microcolonies In vitro biofilm - in depth Ecological succession Inter-bacterial coaggregation Interspecies collaboration - O2 Inter-species competition Inter-species communication Oral ecological zones Mostly the same species present, but proportions differ High biomass sites Non-shedding surfaces Supragingival tooth surfaces Subgingival tooth surfaces Shedding surface The tongue Low biomass (reservoir) sites Shedding oral mucosal surfaces Buccal, palate, external gingiva, floor of mouth Saliva as a transitional zone Subgingival tooth surfaces The tongue Mucosal reservoir sites Smooth exfoliating surfaces How do bacteria keep from being swept away? Some oral species can invade epithelial cells Requires communication between bacteria and cells Bacteria “subvert” the cell to take them in Take control of the cytoskeleton Can live and grow inside Can direct the cell to export them to other cells Multi-species intracellular flora resembles mixed biofilm Invaded buccal cells Collaborative invasion Salivary transport Bacteria during the life cycle Oral colonization begins in the birth canal Reservoir populations on the tongue and mucosa Established during infancy - include anaerobes Tooth eruption provides non-shedding surfaces The “window of infectivity” concept Colonization from reservoir sites and caregiver saliva Hormonal shifts - puberty and pregnancy Can alter propo