Fungal Templates for Noble Metal Nanoparticles.pdfVIP

Supporting Information ? Wiley-VCH 2008 69451 Weinheim, Germany Supporting Information: Fungal Templates for Noble Metal Nanoparticles and their Application in Catalysis Nadja C. Bigall, Manuela Reitzig, Wolfgang Naumann, Paul Simon, Karl-Heinz van Pée, Alexander Eychmüller* Experimental Section In a typical experiment the noble metal nanoparticles were synthesized by a modified Turkevich approach, similar to that of Natan et al.[9]. The metal precursors were gold(III) chloride trihydrate (Sigma- Aldrich, 99.9+ %), chloroplatinic acid hexahydrate (Sigma-Aldrich, ACS reagent) and silver nitrate (Sigma-Aldrich, 99.9999 %). The palladium precursor was prepared by dissolving palladium(II) chloride (Sigma-Aldrich, 99.999 %) in concentrated hydrochloric acid in the ratio PdCl2:HCl 1:2 and by subsequent dilution with deionized water. The final precursor concentration was 0.27 mmol in a total volume of 500 mL in all cases. First, deionized water was heated to boiling followed by addition of the metal precursor. After one minute 11 mL of a solution containing 1 % sodium citrate and 0.05 % citric acid were injected. Another half minute later, 5.5 mL of a freshly prepared sodium borohydrate (0.08 %) solution containing 1 % sodium citrate and 0.05 % citric acid were quickly added and the solution was left to react for 10 minutes. The silver and palladium colloidal solutions were filtered with a hydrophilic syringe filter (pore size 0.22 μm) in order to remove any residual bulk material eventually present. Before inoculation with fungal mycelium, the colloidal solutions were sterilized using a hydrophilic sterile syringe filter with 0.22 μm pore size (Milex-MP). A variety of different fungi consisting of Penicillium citreonigrum, Trametes versicolor, Fusarium, Phanaerochaete crysosporium, Trichoderma viride, Neurospora crassa, Nematolona frowardii, Bjerkandera adusta, and other fungi (in taxation) differing in morphology was selected