A genetic map of large yellow croaker Pseudosciaena crocea.pdfVIP

A genetic map of large yellow croaker Pseudosciaena crocea.pdf

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A genetic map of large yellow croaker Pseudosciaena crocea

(2007) 16–26 /locate/aqua-onlineAquaculture 264A genetic map of large yellow croaker Pseudosciaena crocea Yue Ning a, Xiande Liu a, Zhi Yong Wang a,?, Wei Guo a,b, Yiyun Li a, Fangjing Xie a a The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen 361021, China b Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China Received 14 July 2006; received in revised form 25 December 2006; accepted 30 December 2006Abstract Genetic linkage maps were constructed for large yellow croaker Pseudosciaena crocea (Richardson, 1846) using AFLP and microsatellite markers in an F1 family. Five hundred and twenty-three AFLP markers and 36 microsatellites were genotyped in the parents and 94 F1 progeny. Among these, 362 AFLP markers and 13 SSR markers followed the 1:1 Mendelian segregation ratio (PN0.05). The female genetic map contained 181 AFLP and 7 microsatellite markers forming 24 linkage groups spanning 2959.1 cM, while the male map consisted of 153 AFLP and 8 microsatellite markers in 23 linkage groups covering 2205.7 cM. One sex linked marker was mapped to the male map and co-segregated with the AFLP marker agacta355, suggesting an XY-male determination mechanism and this may be useful in the breeding of monosex populations. ? 2007 Elsevier B.V. All rights reserved.Keywords: Pseudosciaena crocea; AFLP; Microsatellite; Genetic linkage map; Sex determination1. Introduction Genetic maps have become essential tools in many fields of genetic studies and have been constructed in various organisms (Postlethwait et al., 1994; Dib et al., 1996; Dietrich et al., 1996; Groenen et al., 2000), in- cluding several aquaculture species (Kocher et al., 1998; Young et al., 1998; Sakamoto et al., 2000; Robison et al., 2001; Waldbieser et al., 2001). These maps have been efficiently used for various biological analyses, such as quantitative trait loci (QTL) (Jackson et al., 1998; Sakamoto et al., 1999; Ozaki et

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