Airways Lung Diseases.pdfVIP

Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases Elizabeth Fireman,1,7 Moshe Shtark,1 Israel E. Priel,2,3 Robert Shiner,4 Ram Mor,1 Shmuel Kivity,1 and Zvi Fireman5,6 Abstract—We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough 6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20_ inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H202 was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H202. Pulmonary function tests were performed by conventional methods. H202 levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H202 in EBC for each group and for all patients combined. KEY WORDS: exhaled breath condensate; induced sputum; chronic inflammatory lung diseases; biomarker. INTRODUCTION Oxidative stress is the hallmark of various chronic inflammatory lung diseases. Increased concentrations of reactive oxygen species (ROS) in the lungs of affected individuals are reflected by elevated concentrations of oxidative stress markers in the breath, airways, lung tissue and blood [1]. Measurement of these biomarkers has involved invasive procedures to procure the samples or to examine the affected compartments, to the patient_s discomfort. Induced sputum (IS), proving to be a safe and reproducible method has been used to investigate airway inflammation, providing information on both the inflammatory cell