Naturalantisensetranscriptsenhanceboneformationbyincreasingsense.pdf

Bone 51 (2012) 933–938 Contents lists available at SciVerse ScienceDirect Bone j ourna l homepage: www.e lsev ie r .com/ locate /boneOriginal Full Length Article Natural antisense transcripts enhance bone formation by increasing sense IFITM5 transcription☆ Yunshan Liu a,b, Hui Liu a,c, Louisa Titus a,?, Scott D. Boden a,d a Atlanta Veterans’ Affairs Medical Center and Emory University School of Medicine, 1670 Clairmont Rd., Decatur, GA, 30033, USA b Department of Orthopaedics, Emory University School of Medicine, 1670 Clairmont Rd, Decatur, GA 30033, USA c Department of Spine Surgery, The First Affiliated Hospital, Sun Yat-sen University, NO. 58, Zhongshan 2nd Rd., Guangzhou, 510080, China d Emory Orthopaedic and Spine Center, 59 Executive Park South, Suite 3000, Atlanta, GA 30320, USA☆ This work was performed at Atlanta Veterans Affai ? Corresponding author at: VAMC-Research Service, 1 30033, USA. Fax: +1 404 417 1536. E-mail addresses: yliu5@ (Y. Liu), hippocr ftitus@ (L. Titus), sboden@ (S.D. B 8756-3282/$ – see front matter. Published by Elsevier I /10.1016/j.bone.2012.07.024a b s t r a c ta r t i c l e i n f oArticle history: Received 25 May 2012 Revised 13 July 2012 Accepted 16 July 2012 Available online 2 August 2012 Edited by: Thomas Einhorn Keywords: Interferon induced transmembrane proteins Natural antisense transcripts Osteoblast differentiation Bone formationInterferon induced transmembrane protein 5 (IFITM5) has been recognized as an osteoblast differentiation factor. Its regulation, however, is still unclear. In this report, four novel naturally occurring antisense transcripts of rat IFITM2 and IFITM5 transcribed from the opposite strand of the IFITM gene locus, were isolated and characterized. They are alternatively transcribed from rat chromosome 1 and expressed at relatively high levels during early dif- ferentiation of primary isolates of rat osteoblast cells. There are two common fragments in all of the isoform cDNA sequences that are complimentar