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Immune effects of bathing European eels in live
pathogenic bacteria, Aeromonas hydrophila
Guo Song-Lin, Feng Jian-Jun, Yang Qiu-Hua, Guan Rui-Zhang, Wang Yu Lu Pan-Pan
Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technology of the Ministry of
Education, Jimei University, Fujian, Xiamen, 361021, China
Correspondence: Dr R Z Guan, Fishery College of Jimei University/Engineering Research Center of Modern Eel Industrial Technol-
ogy of the Ministry of Education, PRC. Jimei University, Fujian, Xiamen 361021, China. E-mail: rzguan@jmu.edu.cn
Abstract
The specific and non-specific immune parame-
ters and protection of European eels (Anguilla
anguilla) were evaluated after bathing eels with
Aeromonas hydrophila. Two hundred eels were
distributed into two equal groups and bathed
with Phosphate-buffered saline (Control group)
or 1.0 9 107 cfu mL1 A. hydrophila (Test
group) for 1 h respectively. Then, eels were
bled aseptically from the caudal sinus on 1, 4,
7, 14 and 28 days post treatment. The blood
cells were used to evaluate the cellular immu-
nity and the serum was used to determine the
titres of specific antibody as well as the activi-
ties of superoxide dismutase (SOD) and lyso-
zyme. Eels from both groups were challenged
by intraperitoneal injection of 1.0 9 107 cfu of
A. hydrophila on 28 and 42 days post bathing.
The results show that eels bathed in A. hydro-
phila significantly (P 0.05) enhanced the pro-
liferation of different types of blood cells and
the serum titres of anti-A. hydrophila antibody.
The Relative Percent Survival (RPS) after chal-
lenge on 28 and 42 days post bathing in Test
group vs. Control group was 40% and 50%
respectively. These results suggest that bathing
European eels in A. hydrophila would positively
affect specific as well as non-specific immune
parameters and protect against A. hydrophila
infection in freshwater farming.
Keywords: Anguilla anguilla, Aeromonas hydro-
phila, Bathing, Relative pe
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