bd袁媛-杨兆春-中药资源.docVIP

不同来源黄芩种子抗旱特征比较 杨兆春，黄璐琦，袁媛* 通讯作者：袁媛，yyuan0732@ ，陈敏，马文军，李娜 （中国中医科学院中药研究所，100700，北京） 摘要：目的 以20%PEG6000溶液模拟干旱胁迫条件，对不同来源黄芩种子的抗旱特征进行研究。方法 用20%PEG6000溶液分别处理8种来源的黄芩种子8小时以上，对种子的萌发率、萌发指数、各器官的长度进行测定。利用SPSS13.0 for Windows 统计分析软件进行不同来源种子抗旱性的分级。 选择抗旱性差异较大的黄芩种子进行PEG处理，对其保护酶（过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶、超氧化物歧化酶）活性进行分析。结果 模拟干旱条件下，不同来源黄芩种子的萌发状态、生长状况存在差异；聚类分析结果表明且5号材料的抗旱性最强，而1号材料的抗旱性最弱；1号材料CAT活性显著提高，是对照的2.73倍。结论 不同来源黄芩种子抗旱特征存在差异。 关键词：黄芩；种子；干旱胁迫 Comparing of characteristic drought-resistant between different regions YUAN Yuan, YANG Zhaochun, MA Wenjun, LI Na (Institute of Chinese Materia Medica, China academy of Chinese medical science, Beijing, 100700) Abstract: Objection: Simulate solution drought stress conditions with 20% PEG (6000) to research characteristic drought-resistant of Scutellaria baicalensis Georgi seeds from different regions in response to drought stress. Method: The seeds of Scutellaria baicalensis Georgi from different regions was treated with 20% PEG respectively, measure germination rate, germination index, the fresh weight of seedlings and the length of the organs. The seeds were classified by software SPSS13.0 for Windows. And we analyze the activities of protective enzymes of two seeds, which have significant difference. Result: PEG treatments have different influence on the germination rate and the growth of the seedlings of Scutellaria baicalensis Georgi from different regions. Clustering results showed No.5 is drought-resistant, but No.1; the activity of CAT in No.1 was significant increase after stress. Conclusion: The characteristic drought-resistant between different regions could be different. Key words: Scutellaria baicalensis Georgi，seed，drought dress 黄芩Scutellaria baicalensis Georgi为唇形科多年生草本植物。以根入药，为常用中药，需要量较大[1]。黄芩野???资源在我国各地都有分布，近年开始家种生产，河北、陕西、山东等地栽培较多，且多在旱地种植。黄芩播种时由于缺水易发生出苗不齐的现象。PEG6000是一种常用模拟干旱条件的化学试剂，Gergeley[2]用PEG 诱导水份胁迫与对土壤控水的结果差不多，并认为110g 的PEG4000 培养液相当于土壤田间持水量为75％下的水份状况。本研究组以PEG(6000)溶液模拟干旱胁迫条件，研究黄芩种子的萌发和幼苗生长对干旱胁迫的响应。结果表明，20%PEG处理8hr以上可作为黄芩种子干旱模拟条