中药物质基础研究思路与方屠鹏飞中药物质基础研究思路与方法_屠鹏飞.pdfVIP

中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 2 期 2012 年 2 月 ·209· ·专 论· 中药物质基础研究思路与方法 屠鹏飞 1, 2 *，史社坡 1，姜 勇 2 1. 北京中医药大学 中药现代研究中心，北京 100029 2. 北京大学药学院 天然药物及仿生药物国家重点实验室，北京 100191 摘 要：中药物质基础研究是中药现代研究的基础。近年来，随着现代分析、分离技术的不断发展，中药物质基础研究取得 了较大进展，新思路和新方法不断涌现。为了促进中药物质基础研究和基于中药、天然药物资源的活性先导化合物及创新药 物的发现，对当前中药物质基础研究的思路和方法进行综述，并重点介绍基于二维高通量制备液相色谱（2D-pHPLC）技术 的中药化学成分高效分离技术、基于液相-质谱-数据库（LC-MS-DS）/制备液相色谱（pHPLC）技术的中药化学成分快速识 别与高效制备技术、基于 LC-DAD-MSn 技术的中药化学成分研究与数据库的构建等中药物质基础研究的新方法、新技术， 以供参考。 关键词：中药物质基础；天然产物；二维制备液相色谱；液相-质谱-数据库联用技术；液相质谱联用 中图分类号：R284 文献标志码：A 文章编号：0253 - 2670(2012)02 - 0209 - 07 Strategies and approaches on exploring material basis of Chinese materia medica TU Peng-fei1, 2, SHI She-po1, JIANG Yong2 1. Modern Research Center of Beijing University of Traditional Chinese Medicine, Beijing 100029, China 2. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China Abstract: To study the material basis of Chinese materia medica (CMM) is the basis of modernization of CMM. Recently, along with the development of modern analytical methods and separation technologies, material basis research of CMM has achieved great progress, and new strategies and approaches are emerging. In order to promote the material basis research of CMM and the discovery of leading compounds and novel drugs stemmed from CMM and natural products, the present review summarized the current strategies and approaches on the material basis research of CMM. Especially, new strategies and approaches, including rapid separation technology based on two dimensional high-throughput preparative liquid chromatography (2D-pHPLC), rapid identification and high efficient preparation technologies based on liquid