Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispen.pdfVIP

下载本文档

4
0
约5.63万字
约 11页
2017-04-13 发布于江苏
举报
版权申诉

Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispen.pdf

1、本文档共11页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispen

Human colon cancer cells lacking Bax resist curcumin-induced apoptosis and Bax requirement is dispensable with ectopic expression of Smac or downregulation of Bcl-XL Ramachandran Rashmi, Santhosh Kumar and Devarajan Karunagaran Cancer Biology Laboratory, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala 695 014, India To whom correspondence should be addressed. Tel: t91 471 2347975; Fax: t91 471 2348096; E-mail: dkarunagaran@ Multiple apoptotic stimuli induce conformational changes in Bax, a proapoptotic protein from the Bcl-2 family and its deficiency is a frequent cause of chemoresistance in colon adenocarcinomas. Curcumin, a dietary compound from turmeric, is known to induce apoptosis in a variety of cancer cells. To understand the role of Bax in curcumin- induced apoptosis we used HCT116 human colon cancer cells with one allele of Bax gene (Baxt/) and Bax knockout HCT116 (Bax/) cells in which Bax gene is inactivated by homologous recombination. Cell viability decreased in a concentration-dependent manner in Baxt/ cells treated with curcumin (0--50 mM) whereas only minimal changes in viability were observed in Bax/ cells upon curcumin treatment. In Bax/ cells curcumin-induced activation of caspases 9 and 3 was blocked and that of caspase 8 remained unaltered. Curcumin-induced release of cyto- chrome c, Second mitochondria derived activator of cas- pase (Smac) and apoptosis inducing factor (AIF) was also blocked in Bax/ cells and reintroduction of Bax, down- regulation of the antiapoptotic protein Bcl-XL by antisense DNA as well as the overexpression of Smac, highly sensit- ized the Bax/ cells toward curcumin-induced apoptosis. There was no considerable difference in the percentage of apoptotic cells in Bak RNAi transfected Baxt/ or Bax/ cells treated with curcumin when compared with their corresponding vector transfected cells treated with curcu- min. The present study demonstrates the role of Bax but not Bak as a critical regulat