Members of the LBD Family of Transcription Factors Repress.pdfVIP

1 LBD 3 LBD 4 LBD 5 LBD 6 LBD 8 LBD 10 LBD 12 LBD 13 LBD 15 LBD 16 LBD 17 LBD 18 LBD 21 LBD 23 LBD 25 LBD 29 LBD 30 LBD 32 LBD 33 LBD 35 LBD 37 LBD 38 LBD 39 LBD 40 LBD 41 LBD 42 Fo ld in du ct io n 0 5 10 15 20 25 30 min KCl readdition to N-limited seedlings 30 min KNO3 readdition to N-limited seedlings Full nutrition vs. N-limitation 3 h KNO3 readdition Full nutrition vs. P-limitation Full nutrition vs. S-limitation Full nutrition vs. CHO-limitation Supplemental Figure 1. Nitrate-specific, strong induction of LBD37, LBD38, LBD39. Shown are induction levels of all LBD genes represented on the ATH1 GeneChip? (data from Scheible et al., 2004; Morcuende et al., 2007, Osuna et al., 2007, data for sulfur-depletion unpublished). The bars depict the change of transcript level in N-limited seedlings 30 min after re-addition of 3 mM KCl (white hatched), 30 min after re-addition of 3 mM KNO3 (light yellow), 3 h after re-addition of 3 mM KNO3 (full yellow), and of nutrient-replete seedlings versus N- limited seedlings (dark yellow). Orange, blue and cyan bars show the change in the comparison of nutrient-replete seedlings versus carbohydrate (CHO), sulfur (S) and phosphorous (P)-limited seedlings, respectively. Data are from two biological replicates for each condition. Mean values and standard deviations (n=4) are shown and were calculated from crosswise comparisons of the biological replicates (cond. A repl 1 vs. cond. B repl. 1; cond. A repl 2 vs. cond. B repl. 1; cond. A repl 2 vs. cond. B repl. 1; cond. A repl 2 vs. cond. B repl. 2). Supplemental Data. Rubin et al. (2009). Members of the LBD Family of Transcription Factors repress Anthocyanin Synthesis and affect additional Nitrogen Responses in Arabidopsis. 2 WT WT lbd37-2 lbd37-2 lbd39 lbd39 lbd38 lbd38 LBD38OX WT LBD39OX WT LBD39OXLBD38OXLBD37OX2LBD37OX1WT A B C D LBD37OXWT 4 C 6% sucrose P-starvation Supplemental Figure 2. Anthocyanin pigmentation phenotypes. (A) Nine-day-old, N-limited WT an