小麦粉中过氧化苯甲酰的定性和定量检测方法研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.1 小麦粉中过氧化苯甲酰的定性和定量检测方法研究 张玉荣，张德伟，周显青，刘月婷 （河南工业大学粮油食品学院，河南郑州 450001） 摘要：基于过氧化苯甲酰（BPO）在酸性加热条件下可氧化 Fe2+ 生成 Fe3+ ，Fe3+ 与二安替比林甲烷（DAPM ）形成橙色络合物而 显色的原理，建立了小麦粉中 BPO 快速定性分析的目视比色法和定量分析的分光光度法。研究了缓冲液用量、硫酸亚铁铵用量、DAPM 用量、反应温度和反应时间对显色的影响，并对实验条件进行了优化。结果表明，在最优化实验条件下，显色体系的颜色随 BPO 浓 度的升高而加深，色度值呈规律性变化，且在 BPO 浓度 0 mg/L~12 mg/L 范围内符合比尔定律，线性回归方程为 ΔA=0.036+0.110 C （mg/L）， R2=0.9996，420 nm 处表观摩尔吸光系数 ε=2.765×104 L/(mol·cm)。该法用于实际样品的分析，目视比色法可快速确定 BPO 的含量范围，分光光度法检出限为 0.016 mg/L，加标回收率为 97.0~101.8%，相对标准偏差(RSD)小于 5%。 关键词：小麦粉；过氧化苯甲酰；快速检测；目视比色法；分光光度法 文章篇号：1673-9078(2014)1-170-174 Qualitative and Quantitative Detection of Benzoyl Peroxide in Wheat Flour ZHANG Yu-rong, ZHANG De-wei, ZHOU Xian-qing, LIU Yue-ting (College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China) Abstract: Based on the principle that Fe2 + is oxidized to Fe3+ by benzoyl peroxide (BPO) under acidic and heating conditions, and orange complex was generated by Fe3+ and diantipyryl methane (DAPM), visual colorimetry for fast qualitative analysis and spectrophotometric for quantitative analysis of BPO in wheat flour were built. In this paper, the experimental conditions of BPO detection method were optimized, and the experimental influencing factors including buffer solution content, ammonium ferrous sulfate content, DAPM content, reaction temperature and reaction time were studied. The results showed that un