稳定表达中国人常见GJB2基因突变细胞系的建立.pdfVIP

doi??10??3969??j??issn??1672??4933??201????? 稳定表达中国人常见?? 一张延平“‘张晓强?跹爬?黄玮?镉袢? ZhANG ???? zhA^||G xi????qi??gl??J?一??? ???轧??Ⅻ 【摘要】目的将中国人常见??基因突变?????真核表达载体稳定转染入???细胞，建立稳定 ??????c??235deIc??HEK293??????????????pcx26??EGFP??pcx26??235deIc??EGFP????HEK293??????24h ????G418??????????????FcM????GFP????????????????????????????????????GFP???????????????????? ??????????????????????????????????????????????pcx26??EGFP??Pcx26??235deIc??EGFP??HEK293?? 胞都能够检测到??牡鞍滋醮??晕⒕迪驴杉?榷ū泶锘?虻腍???赴?毡楸泶颎?。结论本研究 成功构建了稳定表达中国人常见??基因突变的???细胞系。为今后的研究工作打下基础。 【关键词】感音神经性耳聋；缝隙连接；突变 【????】?正???籲，．，童? construct the ??? ?¨¨? ??? ????? ?? ???? ??? ? ????甞，?打??? ?×?一?? and ???????—?? ?? ??§??? ?? ??? ce??s??Twenty??fOurhours ???瑃? ceIIs ?? d??utedand G41 8 was added ? ??． FCM ?¨??? was ?? ? ??? pos??VeGFP ce??s2 weeks after ?耼????．??，??? ?? and laser ???? ????? ?? app??ed? ???? the ????? ? c??235deIC????r_??_?? The western ?? resuIts ??? ?? GFP was expressed ? the ??? ceIIs transfected ?? pCx26??EGFPand ???????狤??甌? ????????? ????? ???? ? w??Id?? ?? ?? ? the ?¨???? and ????? ???? ? the cytopIasm can ? ?? under the ???? micrOscope??C??c??I??z????The??? ?¨¨? ??? ????? ?? ???? ??? ? Chinese has ?? ?????? estab??shed??thusbeing ?? ? proVide a new ?? ? further explore the mechanism ? ??猻???? hea ?? ?????． ??Key???縎?????? hearing Ioss??Gap????；???? G J B ??虮嗦敕煜读?拥鞍? 6 ·基金项目：全军”十一五”项目归国人员课题????； ??????琧??基因突变是导致人类耳聋的主要