百日咳手册.pdf

百日咳鲍特菌/副百日咳鲍特菌 实验室诊断手册 目录 缩写.................................................................................................................... v 致谢 .......................................................................................................... vii 1. 前言 .............................................................................................................. 1 2. 百日咳实验室诊断总括 ................ 6 3. 直接诊断........................................................................................................ 7 4. 间接诊断 ................................................................................................... 1 1 5. 参考书目 ............................................................................................................ 1 2 附录 1: 咽拭子的收集 (NPA) ................................... 1 4 附录 2: RL 培养基制备 ............................................................................ 1 6 附录 3: B-G 培养基制备 .......................................................................... 1 9 附录 4: 碳血琼脂培养基制备 .......................................................................... 2 2 附录 5: 革兰染色 ..................................................................... 2 5 附录 6: Kovac’s 氧化酶实验, 硝酸盐还原酶实验,尿素酶实验, 碳水化合物利用实 验 ......................................... 2 7 附录 7: 免疫荧光检测 ............................................................................... 3 0 附录 8: 百日咳分型 ........................................................................35 附录 9: 鲍特菌储存 ........................................................ 37 附录 10: 百日咳插入序列 IS481 荧光探针的实时 PCR....................................................................................40 附录 11: 副百日咳插入序列 IS1001 荧光探针的实时 PCR.............................................................................