红高粱色素的提取工艺及结构的初步分析.pdfVIP

中国农学通报 2010,26(17):79-85 Chinese Agricultural Science Bulletin 红高粱色素的提取工艺及结构的初步分析 李竹英 1，毛绍春 1，戴荣珍 2，孙桂兰 1，谢 旭 1 （1玉溪农业职业技术学院，云南玉溪 653100；2玉溪市红塔区种子管理站，云南玉溪 653100） 摘 要：研究高粱色素的最佳提取工艺和结构，为开发和应用提供实验数据。利用回流提取法分别考察 4 了浓度、酸度、提取时间、温度等因素对红高粱色素提取率的影响，通过正交实验（L93）筛选红高粱色素 提取的最佳工艺；利用光谱法研究了红高粱和紫黑高粱色素溶液在波长 180~800 nm 范围内的光谱数 ℃ 据。正交实验确定的红高粱色素最佳提取工艺是加热温度为 80 、浓度为 75% pH=2 的乙醇溶液、固液 比为1:100、加热时间为2 h。红、紫黑两种高粱壳色素溶液在可见光区的最大吸收峰波长分别是494 nm 和 499 nm，紫外光谱数据显示红高粱色素含有二氢黄酮或二氢黄酮醇类物质。红、紫黑高粱色素溶液 在可见光区的光谱数据不相同，红、紫黑高粱色素溶液在紫外区光谱特性与黄酮（芦丁）结构的光谱特 性也不相同。 关键词：高粱色素；提取；结构；分析 中图分类号：TS235.2 文献标志码：A 论文编号：2010-1124 Extracted Method and Preliminary Structural Analysis of Red Sorghum Pigment Li Zhuying1, Mao Shaochun1, Dai Rongzhen2, Sun Guilan1, Xie Xu1 ( , Yuxi Yunnan 653100; , Yuxi Yunnan 653100) 1Yuxi Agricultural vocational Institute 2Yuxi City Hongta Seed Station The best extracted method and structure of red sorghum pigment were studied to provide Abstract: experimental date for the development and application. The effect factors of extracted rate of red sorghum pigment, such as chroma, acidity, temperature and extraction time was tested respectively by refluxing extracted method. The best extracted method of red sorghum pigment was selected by the orthogonal experiment (L 34). Pigment solution of red sorghum and purple black sorghum was scanned at the wavelength 9 range of 180-800 nm. The orthogonal experiment define that the best extraction method of pigment of red sorghum were 80℃,75