草鱼肠道组织抑制性消减表达序列标签的初步分析.pdfVIP

第 36 卷第 3 期 湖南农业大学学报(自然科学版) Vol . 3 6 No.3 2010 年 6 月 Journal of Hunan Agricultural University (Natural Sciences) Jun．2010 DOI:10.3724/SP.J.1238.2010.00339 草鱼肠道组织抑制性消减表达序列标签的初步分析 章卓1，雷红宇2，苏建明2* (1.全国畜牧总站，北京 100125；2.湖南农业大学 动物医学院，湖南 长沙 410128) 摘 要：以致病性嗜水气单胞菌(Aeramonas hydrophila)人工感染的草鱼肠道组织为材料，采用抑制性消减杂交技 术，构建了草鱼肠道组织消减 cDNA 文库，对文库克隆的序列测定和初步生物信息学分析，共获得 147 个草鱼肠 道表达序列标签(EST)．序列???源性搜索结果表明，97 个 EST 代表了 46 个已知基因，其余 50 个 EST 为未知序列．与 细胞防御相关的 EST 有 43 个，分属 13 个基因． 关键词 ：草鱼；抑制性消减杂交；cDNA 文库；表达序列标签 中图分类号：Q785：S917 文献标志码：A 文章编号：1007-1032(2010)03-0339-05 Preliminary analysis of expressed sequence tags(ESTs) of subtracted cDNA library from intestine of grass carp ZHANG Zhuo1, LEI Hong-yu2, SU Jian-ming2* (1.National Station of Animal Husbandry, Beijing 100125, China; 2.College of Veterinary Medicine, HNAU, Changsha 410128, China) Abstract：The intestine of fish plays an important role in carbohydrate, protein, lipid metabolism and immunity. To obtain molecular biological information to the studies of some important physiological procession in grass carp. A subtractive cDNA library was constructed from the intestinal tract of grass carp experimentally infected with Aeromonas hydrophila by suppression subtractive hybridization (SSH). A total of 211 cDNA clones were partially sequenced and 147 ESTs obtained from the library. BLAST result showed that 147 of the intestinal ESTs could be ascribed to the transcri