人试剂盒,乳酸脱氢酶(LDH) ELISA 试剂盒说明书.doc

人试剂盒,乳酸脱氢酶(LDH) ELISA 试剂盒说明书 Kit, lactate dehydrogenase (LDH) ELISA kit instructions  1200μg/L5号标准品150μl的原倍标准品加入150μl标准品稀释液600μg/L4号标准品150μl的5号标准品加入150μl标准品稀释液300μg/L3号标准品150μl的4号标准品加入150μl标准品稀释液150μg/L2号标准品150μl的3号标准品加入150μl标准品稀释液75μg/L1号标准品150μl的2号标准品加入150μl标准品稀释液人试剂盒,乳酸脱氢酶(LDH) ELISA 试剂盒说明书 远慕技术说ELISA法是一种具有敏感性高,特异性强,重复性好等优点的实验诊断方法，由于其试剂稳定、易保存,操作简便,结果判断较客观等因素,已广泛应用在免疫学检。elisa试剂盒操作步骤1. 从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。2. 设置标准品孔、样本孔和空白孔，空白孔什么都不加；标准品孔各加不同浓度的标准品50μL；3. 待测样本孔先加待测样本10μL，再加样本稀释液40μL；4. 随后标准品孔和样本孔中（空白孔不加）加入辣根过氧化物酶（HRP）标记的检测抗体100μL，，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。5. 弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。乳酸脱氢酶(6. 所有孔加入底物A、B各50μL，37℃避光孵育15min。7. 所有孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。 人试剂盒,乳酸脱氢酶(LDH) ELISA 试剂盒说明书Kit is solid clip art experiment enzyme-linked immunosorbent (ELISA). The known standard, the concentration of unknown sample which concentration of adding the microporous enzyme label plate for testing. The first and biotin labeled antibody incubate at the same time. After washing, add avidin tagged HRP. Again after incubate and washing, the enzyme that removes not combine, then add in the substrate of A and B, and the enzyme combination at the same time. Color is produced. The depth of the color and the sample concentration in proportional relationship.乳酸脱氢酶 人试剂盒,乳酸脱氢酶(LDH) ELISA 试剂盒说明书 ELISA kit product species distribution: Poultry: chicken, duck, goose, etc Birds: pigeons and birds, etc Aquatic products: fish, bullfrog, etc Animal: mice, elephants, tiger, sheep, horse, tadpole, toad, cattle, the panda, the golden mice, pigs, rabbits, lizards, hippos, rhesus monkeys and apes and baboons, deer, etc. Indirect ELISA kit the simple steps: (1), the specific antigen and solid phase carrier, the formation of solid-phase antigen; (2), and dilution of serum tested, thermal reaction. Specific antibody in the serum and solid-phase antigen, the formation of solid-phase antigen complex anti - body; (3), plus