Eag1钾离子通道在宫颈癌中的表达以及缺氧对其的调控的论文.docVIP

　　Eag1钾离子通道在宫颈癌中的表达以及缺氧对其的调控的论文 eag1钾离子通道在宫颈癌中的表达以及缺氧对其的调控 【关键词】 宫颈癌;钾离子通道;eag1 （本站文章仅给需要的医务工作者提供交流学习参考。翰林医学免费提供。部分资源由工作人员网上搜索整理而成，如果您发现有哪部分资料侵害了您的版权，请速与我们后台工作人员联系，我们将即时删除。客服qq后台工作qq：928333977） 【摘要】 目的 研究不同病变宫颈组织中eag1表达情况以及缺氧对宫颈癌hela细胞膜上eag1钾离子通道表达和活性的影响。方法 采用免疫组化法检测不同宫颈组织和宫颈癌hela细胞中eag1蛋白的表达情况;采用rt-pcr法检测不同缺氧时间和缺氧浓度对hela细胞中eag1 mrna表达的影响。采用全细胞膜片钳记录的方法，研究缺氧对eag1钾离子通道活性的影响。结果 宫颈上皮内瘤变组织中eag1蛋白的表达明显高于正常组，宫颈癌组织中eag1的表达明显高于宫颈上皮内瘤变组;eag1的表达随临床分期的增加逐渐增高，eag1的表达与病理分级无明显相关性。正常培养的宫颈癌hela细胞中即有eag1表达;其表达随缺氧程度加深及缺氧时间的延长明显增强。hela细胞中eag1钾离子通道是电压依赖性的，具有延迟整流及不失活特性;轻度缺氧条件能使hela eag1钾离子通道电流强度增大、激活时间缩短。.结论 eag1钾离子通道蛋白有可能成为新的早期筛查和判断宫颈癌生物学行为的诊断指标。 【关键词】 宫颈癌;钾离子通道;eag1 　expression of eag1 k+ channel in cancer of the cervix and the regulation of hypoxia 　　lin shuang,li li,liu yuan,et al. department of obstreics and gynecology,daping hospital,the third military medical university,chongqing 400042,china 　　[abstract] objective immunohistochemistry ployed to detect the expression of eag1 protein in different cervix uteri tissues and cacx hela cells.methods ined the effect of different hypoxia time and hypoxic concentration on the expression of eag1 mrna in hela cell using rt-pcr. meanp recording ployed to investigate the effect of hypoxia on activity of eag1 k+ channel.results the expression of eag1 protein in cervical intraepithelial neoplasia (cin) tissue al control, and the expression in cacx tissue arkably higher than that in cin group. the expression of eag1 ascended as the development of clinical stage of cervical cancer, but almost irrelevant to pathological grade. results of immunofluorescence and rt-pcr suggested that the expression of eag1 significantly increased along e. p recording shoent cell membrane potential of hela, increase the current intensity of eag1 k+ channel and shorten the activation time.conclusion eag1 k+ channel protein is possible to bee diadynamic criteria for neent. meanportant role in hypoxic reception of cacx hela cell. 　　[key channels; eag1 　　宫颈癌是