丹芪合剂对DM大鼠肾小管BMP.docVIP

　　丹芪合剂对DM大鼠肾小管BMP 作者：肖瑛，石明隽，桂华珍，郭兵，张国忠 【摘要】 　　目的观察丹芪合剂和依那普利对糖尿病（dm）大鼠肾小管上皮细胞骨形态发生蛋白-7（bmp-7）及其受体alk-3表达的影响，探讨丹芪合剂的药理机制。方法将sd大鼠分为正常对照组（a组）、糖尿病组（b组）、糖尿病丹芪合剂组（c组）和糖尿病依那普利组（d组）。以链脲佐菌素复制dm大鼠模型。于实验12周处死大鼠后测定相应生化指标和肾脏指数，pas染色观察肾组织的形态结构变化；免疫组化检测肾小管上皮细胞bmp-7和fn蛋白的表达；rt-pcr方法检测肾皮质bmp-7和alk-3 mrna水平。结果①b组的血糖、甘油三酯和总胆固醇、血肌酐、肾脏指数均显著高于a组并出现明显的蛋白尿，c和d组上述指标除血糖外均明显低于b组。②免疫组化结果显示bmp-7蛋白高表达于a组肾小管上皮细胞胞浆，fn仅有少量表达；b组bmp-7蛋白的表达明显减少，fn表达却明显增多；c和d组肾小管上皮细胞bmp-7蛋白表达显著高于b组，但低于a组，同时fn的表达减少。③rt-pcr结果显示，与a组相比，b组肾皮质bmp-7表达明显减少，且alk-3 mrna未见表达，c和d组bmp-7及其受体alk-3 mrna表达均显著高于b组，d组alk-3 mrna表达甚至高于a组。.结论丹芪合剂和依那普利可能通过促进内源性bmp-7及其受体alk-3的表达,使fn的沉积减少,从而对糖尿病大鼠肾脏起保护作用。 【关键词】 糖尿病肾病； 骨形态发生蛋白-7； alk-3； 丹芪合剂； 依那普利 　　abstract：objectiveto investigate the effects of dan qi mixture and enalapril on the expression of bone morphogeic protein-7(bmp-7) and its receptor alk-3 in the renal tubules of diabetic rats. methods sd rats ly divided into normal control group (group a), diabetic group (group b), diabetic danqi-treated group (group c), diabetic enalapril-treated group (group d). the diabetic rat model ade ical methods. kidney index orphology ined in pas staining sections. immunohistochemistry ployed to detect the expression of bmp-7 and fn protein in renal tubules of all groups. the mrna levels of bmp-7 and its receptor alk-3 in renal cortex ined by rt-pcr. results①pared arkably in group b. in group c and d, parameters above-mentioned except blood glucose p-7 protein munohistochemistry in group a. on the other hand, the protein of bmp-7 arkably in group b, and the protein of fn p-7 protein p-7 mrna and its receptor alk-3 decreased remarkably by rt-pcr in group b. in group c and d, the expression of the bmp-7 and alk-3 mrna rna in group d ixture and enalapril can promote the expression of endogenous bmp-7 and its receptor alk-3 in the renal tubule cells, and therefore reducts the deposition of fn and protects the kidney of diabetic rats. 　　key p-7； alk-3； danqi mixture； enalapril 　　糖尿病肾病是导致终末期肾衰竭的常见原因，目前尚无有效的治疗