云南白药对自发性高血压大鼠肾脏炎症状态的改善作用的论文.docVIP

　　云南白药对自发性高血压大鼠肾脏炎症状态的改善作用的论文 作者：田登科， 陈刚领， 李亚娟， 胡送友， 刘俊，卞卡 【摘要】 目的观察云南白药(ynby)对自发性高血压大鼠(shr)肾脏炎症状态的影响，探讨其对高血压肾脏疾病的防治作用及机制。方法将6周龄shr分为ynby治疗组和高血压模型对照组，在3个时间点(给药6周，给药14周，停药后9周)研究ynby对shr血压值、肾脏炎性因子表达和氧化应激水平的影响。结果ynby对shr无明显降压作用(pgt;0.05)。ynby在mrna和蛋白水平抑制了细胞间粘附分子-1(icam-1)和内皮型一氧化氮合酶(enos)在shr肾脏的高表达(plt;0.05)。 ynby在给药14周和停药9周时降低了过氧化物酶体增殖物活化受体γ(pparγ)的mrna表达(plt;0.05)，在给药14周时明显降低了pparγ的蛋白表达(plt;0.05)。ynby给药后显著降低了shr肾脏组织蛋白羰基化水平(plt;0.05)，在给药14周及停药9周后均可显著提高shr肾脏总抗氧化能力(plt;0.05)。结论ynby给药后能够显著减轻shr肾脏炎症反应和氧化应激水平，ynby可能在防治高血压肾病方面具有一定的疗效。 【关键词】 云南白药; 自发性高血压大鼠; 高血压肾脏疾病; 炎症反应 　　abstract：objectiveto investigate the effects of yunnanbaiyao(ynby)on inflammatory status of the kidney from spontaneously hypertensive rats (shr), and to explore preventive and therapeutic usage of ynby on hypertensive nephropathy. methods6 mation-related factors and level of oxidative stress ined at 3 time points: 6 ent; 14 ent; and 9 ent.resultsynby did not affect the elevation of blood pressure of shr(pgt;0.05). ho pound inhibited the expression of intercellular adhesion molecule-1 (icam-1) and endothelial nitric oxide synthase (enos) at both mrna and protein levels (plt;0.05). at the time points of 14 ent and 9 ent, hmp significantly attenuated peroxisome proliferator-activated receptor γ(pparγ) mrna expression (plt;0.05). the protein level of pparγ arkedly after 14 ent (plt;0.05). ynby significantly reduced protein carbonyl contents of shr kidney (plt;0.05). in addition, after 14 ent, ynby increased total anti-oxidant capacity of renal tissue from shr (plt;0.05) and this elevated anti-oxidant capacity aintained for 9 ent (plt;0.05). conclusionynby can reduce the inflammatory status and oxidative stress of shr kidney, ay serve as the base for the advanced therapeutic utility of ynby in hypertensive renal disease. 　　key mation 　　肾脏是高血压的重要靶器官之一。.最近研究表明，炎症反应不仅参与了高血压疾病的发生发展，更是与高血压心、脑、肾等多个靶器官损害密切相关[1]。炎症反应不仅可导致肾脏纤维化和肾单位的损伤，同时也参与了肾小球滤过膜的破坏，因此抗炎相关治疗已经成为防治高血压肾脏疾病新的重要环节。云南白药(ynby)由云南民间医生曲焕章于1902年研制成功，具有活血消肿、止血