缺血后处理对大鼠缺血-再灌注心肌髓过氧化物酶及细胞间粘附分子的论文.docVIP

　　缺血后处理对大鼠缺血/再灌注心肌髓过氧化物酶及细胞间粘附分子的论文 【摘要】 目的：研究缺血后处理对大鼠缺血/再灌注心肌髓过氧化物酶（mpo）及可溶性细胞间粘附分子（sicam）的影响。方法：选择健康sd大鼠48只，随机分为3组：假手术组、缺血再灌注组（对照组）和缺血后处理组，每组16只。制备大鼠心肌缺血再灌注模型。缺血再灌注组,收紧结扎线缺血40 min, 放松结扎线再灌注240 min；缺血后处理组,缺血40 min后, 再灌注10 s,缺血10 s,连续3个循环，然后再灌注240 min；假手术组，开胸后穿线做套环，但不收紧结扎线。再灌注结束后检测血清肌酸激酶（ck）活性、sicam含量及心肌mpo活性。结果：①血清ck活性：试验后缺血后处理组和缺血再灌注组（对照组）的ck活性明显高于假手术组[分别为（736.28±21.72），（987.62±28.58），（256.34±19.34）u/l，plt;0.01], 缺血后处理组的明显低于对照组(plt;0.01)。②心肌mpo活性：缺血后处理组和对照组的均显著高于假手术组（plt;0.01），缺血后处理组的较对照组显著降低［（0.86±0.08）u/g∶（1.28±0.26）u/g，plt;0.01］。③血清sicam含量：缺血后处理组和对照组血清的sicam含量均显著高于假手术组（plt;0.01）。缺血后处理组的较对照组显著降低［（54.28±11.69）ng/ml∶（76.62±13.45）ng/ml，plt;0.01］。结论：缺血后处理可减轻缺血再灌注损伤，其机制可能与减轻氧化损伤、抑制白细胞的粘附有关。. 【关键词】 心肌再灌注损伤；细胞粘附分子；过氧化物酶 　effect of postconditioning on mpo and sicam in rats yocardial ischemia reperfusion/liu shenghui, huo yue, yin boying//chinese journal of cardiovascular rehabilitation medicine,2009,18(2):107 abstract：objective:to study the effect of postconditioning on mpo and sicam in rats yocardial ischemia reperfusion.methods: totally 48 rats ly divided into three groups: control group (n =16):in anesthetized openchest rats, the left anterior descending (lad) coronary artery in and reperfused for 4 h,no intervention for reperfusion; ischemic postconditioning (ip) group (n = 16): after lad occlusion 40 min, three cycles of 10 s reperfusion folloinute of reperfusion,then reperfused for 4 h; sham group (n=16):the surgical procedure creatine kinase(ck),myeloperoxidase (mpo) activity and sicam content easured.results:serum ck activity in ip group po activity and sicam content l∶（76.62±13.45）ng/ml respectively,plt;0.01］.conclusion:ip has protective effect on injury induced by i/r in rats. it has the effect decreasing mpo activity and ameliorating neutrophil adhesion and infiltration. author′s　address：department of cardiology,affiliated hospital of hebei university,baoding,hebei,071000,china key yocardial reperfusion injury;cell adhesion molecule;peroxidase 缺血后处