陈润生07年课件.pptVIP

;; ;BREAKTHROUGH OF THE YEAR(2001): Science celebrates nine other areas in which important findings were reported this year, from subatomic to atmospheric and beyond.First runner-up: RNA ascending. ;;; ; ;Biological Dark MatterNewfound RNA suggests a hidden complexity inside cellsJohn Travis;; ; ;The stem-bulge RNAs of C. elegans ;The snRNA like RNAs of C. elegans ; Confirm three special upstream motifs of noncoding genes—UM1-3;The expression levels of non-motif snoRNAs with the frequencies of ESTs corresponding to exons of their host genes, produced a distinct positive correlation not found for motif-containing loci.; ;Arrangements of transcriptional elements and genomic locations of small non-coding ncRNA loci, as inferred from genomic and experimental data. ;Analysis of transcription levels of 106 ncRNA families were carried out with Northern blot. 61 showed variation exceeding two standard variation, composed of 6 distinct expression clusters. ;; ; ; ;2、Mapping the C. elegans noncoding transcriptome with a whole genome tiling microarray;Tiling;Structure of eukaryotic mRNA;RNA was extracted from a mixed stage population of wild type C. elegans strain N2;Build Transfrag;Find TUFs (Transfrag of Unknown Function);Transfrag distribution in the three different samples. “Other annotated” mainly includes tandem repeats and pseudogenes;Detection rates of annotated ncRNAs in the SNPA sample;The NPA sample produced 97,548 transfrags which could potentially all represent non-coding transcripts. Nearly 24% are non-annotated intergenic TUFs. The RT-PCR analysis confirmed 89% (25/29) of randomly sampled intronic and intergenic TUFs, effectively excluding the possibility that the majority of the NPA TUFs are a result of microarray non-specific hybridization. TUFs in the NPA sample are also fairly well conserved, with 54% showing at least some level of conservation (weak WABA (Kent and Zahler, 2000)) in C. briggsae. NPA TUFs are generally short (mean 88 nt, median 7