一株菲降解菌的特性及相关降解基因的克隆.PDF

应用与环境生物学报 2009，15 ( 5 ): 682~685 2009-10-25 Chin J Appl Environ Biol=ISSN 1006-687X DOI: 10.3724/SP.J.1145.2009.00682 一株菲降解菌的特性及相关降解基因的克隆* 何丽娟1 李正华2 洪 青1** 李顺鹏1 (1南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室 南京 210095) (2江苏省宜兴市前程生物有限公司 宜兴 214253) 摘 要 从石油污染土壤中分离到一株菲降解菌2F5-2. 根据该菌株生理生化特征和16S rDNA序列相似性分析，将其 初步鉴定为鞘氨醇杆菌属(Sphingobium sp.). 该菌株在10 h内对100 mg/L的菲的降解率为100%. 降解菲的最适温度为 30 ℃，最适pH为7. 对降解途径的初步研究显示，该菌株通过水杨酸途径降解菲. 克隆了编码芳香烃双加氧酶α亚基 的基因phdA，它与菌株Sphingomonas sp. P2、Sphingobium yanoikuyae B1、Sphingomonas sp. ZP1中phdA的同源性分别为 97.9%、98%和100%，表明该基因具有保守性. 图6 参16 关键词 菲；生物降解；降解途径；phdA基因 CLC X172 + Q785 Characterization of A Phenanthrene-degrading Strain and Cloning of Degradation-related Gene* HE Lijuan1, LI Zhenghua2, HONG Qing1** LI Shunpeng1 (1Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China) (2Yixing Qiancheng Bio-engineering Co., Ltd., Yixing 214253, Jiangsu, China) Abstract A?????????????????????????????????? bacterial strain 2F5-2 capable o?? degrading?????????????????????? phenanthrene ?w?????????????????????????????????????????????????as isolated ??rom petroleum-contaminated soil. It ?w??as preliminarily identified as Sphingobium sp. according to its physiological biochemical characteristics and the analysis o?? its 16S rRNA gene sequence. Strain 2F5-2 could degrade 100% o?? 100 mg/L phenanthrene within 10 h .The optimal pH and temperature ??or the degradation were 7 and 30 ℃, respecti