1 Artemisia flavonoids macroporous resin adsorption separation Preparation.docVIP

 PAGE \* MERGEFORMAT 14 1 Artemisia flavonoids macroporous resin adsorption separation Preparation Author: hot-Na Kasi wood, Sheng-Ping, Yang Xiaocui [Abstract] Objective To study the enrichment of macroporous resin adsorption process of total flavonoids an Artemisia conditions and parameters. Methods for the study of indicators of total flavonoids, using colorimetry content, visit a macroporous resin concentration of total flavonoids of Artemisia process conditions. The results of an Artemisia extract (0.04 g crude drug / ml) 10 ml on a macroporous resin column (R 15 mm * H 290 mm), adsorption 30 min, the first eluted with 150 ml distilled water, and then, using 150 ml 70% ethanol elution rate of 3 ml / min, eluting agent is 10 times the amount of resin, the resin can be reused three times, using this condition is the best technique. Conclusion HPD-450 macroporous resin determined by process conditions, it can be a better adsorptive separation Artemisia flavonoids. The elution of total flavonoids things up 3.18% or more, the total recovery rate of 86.91% or more flavonoids. [Keywords:] an Artemisia; total flavonoids; macroporous resin Abstract: ObjectiveTo study the optimal conditions and the technological parameters for enrichment process of total flavone of Artemisia rupestris L. with macroporous absorption resin. MethodsWith the enrichment degree of total flavone of Artemisia rupestris s L.. As a marker, the optimal conditions and technological parameters of the enrichment process were investigated. ResultsThe optimum process was as follows: the concentration of extract of water from the Artemisia rupestris L. was 0.04 g / ml; eluting velocity, 3 ml / min; the eluting reagent, 70% ethanol (10 times the volume of the resin); and the HPD-450 resin could be used 3 times repeatedly. ConclusionThe results showed that HPD-450 macroporous resin could well absorb and separate Artemisia rupestris L. total flavone under the set technological conditions, and the c