20 major producing country Quality of analysis and comparison of Poria.docVIP

 PAGE \* MERGEFORMAT 12 20 major producing country Quality of analysis and comparison of Poria Of: Zan Junfeng, Xu Bin, in Wavelets, Su Wei, Wang Keqin, Liu Yan Wen [Abstract] Objective To establish a CMP and Poria acid determination, analysis and comparison of the 20 major producing country in the CMP and Poria cocos acid content, in order to evaluate the quality of different habitats provide the basis for the quality of Poria. Methods Determination of Poria polysaccharide content, alkaline extraction, phenol - sulfuric acid color, glucose as reference, the detection wavelength 490 nm. by RP-HPLC determination of Poria acid, Kromasil 100-5C18 column, column temperature 30 ℃, the mobile phase acetonitrile -0.2% formic acid (80:20), flow rate 1.0 mL / min, detection wavelength 242 nm. The results of glucose in the 40 ~ 200? g / mL range of good linearity, r = 0.999 9, the average recovery was 99.72%, RSD = 0.99%; Poria acid 0.48 ~ 2.40? g within the scope of its chromatographic peak area was linear, r = 0.999 7, the average recovery of 100.26%, RSD = 1.26%. different areas of polysaccharides in Radix and Poria cocos acid quite different levels, indicating that the quality of medicines is not consistent. Conclusion The method is simple, rapid, accurate and reliable for the evaluation of the quality of herbs from different areas Poria provides a reliable method of analysis. [Keywords:] Poria; CMP; Poria acid; spectrophotometry; high performance liquid chromatography Abstract: Objective To establish a method for determining the content of effective ingredient pachymaran and Pachymic acid in Poria, compare the content of pachymaran and Pachymic acid of Poria cocos from different origin places, and provide an evidence to evaluate the quality of Poria cocos. Methods Spectrophotometry method was used for determination of pachymaran, with phenol-sulfuric acid color, glucose as the standard and the detection wave length at 490 nm. RP-HPLC method was used for