40 cases of Kunming High-titer anti-HBc result in false-negative results processing and analysis.docVIP

 PAGE \* MERGEFORMAT 4 40 cases of Kunming High-titer anti-HBc result in false-negative results processing and analysis Of: Chen Qian Hu Yan Zhang Yan Yan Yufen Renfu Xiang Shi Min Jiang Muyao Lv Yujie [Keywords:] Kunming high titer of anti-HBc false-negative treatment guide In recent years, the inspection process, due to hepatitis B core antibody (anti-HBc) high-titer false-negative results caused by reports of increased year by year [1], to further verify the conclusions, this paper 40 cases of Kunming detection of two pairs of semi , anti-HBc-negative patients the results were the corresponding processing, analysis report is as follows. 1 Materials and methods 1.1 The source of specimen samples in this experiment were from all relevant departments in the hospital outpatient and inpatient treatment, the two pairs of semi detected as HBsAg (+), HBeAg (+) and anti-HBc (-) or HBsAg (+) , anti-HBe (+) and anti-HBc (-) modes, of which 20 cases of each type of model, a total of 40 cases. 1.2 The test method of the markers of hepatitis B by ELISA, and the Shanghai Branch of China Industrial Biotechnology Co., Ltd. produced by enzyme-linked immunosorbent assay (ELISA) kit for detection. These two modes of anti-HBc (-) of the test results are 1:100 dilution of serum was obtained after [2]. The test further 1:1000,1:10000 and by 10% 2 - mercaptoethanol (2-ME) treatment (serum 1ml added 2-ME0.2ml) serum, according to conventional competitive inhibition ELISA method for anti-HBc testing. 2 Results and discussion 2.1 The tests detected by the anti-HBc positive result: HBsAg (+), HBeAg (+) and anti-HBc (-) group of 20 cases, of which 0 cases of 1:100, 1:1000 9 cases, 1 : 10000 20 cases ,2-ME 20 例, HBsAg (+), anti-HBe (+) and anti-HBc (-) group of 20 cases, of which 0 cases of 1:100, 1:1000 in 7 cases, 1: 10000 18 cases ,2-ME 18 cases. The results can be seen from the high dilution of the positive group than the low number of patients dilution group, is