3 - Daidzein Sulfonate on prostate cancer cells and the effects of PTEN gene.docVIP

 PAGE \* MERGEFORMAT 7 3 - Daidzein Sulfonate on prostate cancer cells and the effects of PTEN gene Authors: Zeng Jing, Song Tao, Zhi-Hua Huang, Xiao, Huang Zhen [Abstract] Objective To observe the 3’-daidzein sulfonate on prostate cancer cells (PC-3) proliferation and gene expression to explore the prevention of prostate cancer new drugs. Methods PC-3 in F12 medium (containing 10% fetal calf serum) in an open adherent monolayer culture, experimental set up solvent control group, six subjects each dose group objects, using MTT method, PC-3 in proliferation analysis, pairs of 3’-daidzein sulfonate after the role of androgen-independent prostate cancer (PC-3) cell viability, cell toxicity was studied. The results with double distilled water control group, 3’-daidzein sulfonate on PC-3 cells in a dose-dependent inhibitory effect, can induce prostate cancer (PC-3) apoptosis and necrosis caused by effects at the same time induce PC-3 cells (PTEN gene) expression. Conclusion 3’-daidzein sulfonate has significantly inhibited the proliferation of PC-3 prostate cancer effects, and dose-dependent manner. [Keywords:] 3’-daidzein sulfonate PC-3 cells, PTEN gene Prostate cancer is a hormone associated with the infiltration of a strong male cancer, its incidence and mortality rates are high. Prostate cancer in the transformation of a non-hormone-dependent prostate cancer, the no effective treatment. Therefore, to find effective, economic security precautions has become a pressing public health problem worldwide. In prostate cancer research and treatment of the mechanism, PTEN (phosphatase and tensin homology deleted on chromosometen) gene has attracted wide attention, PTEN is a newly discovered on chromosome 10q23.3, with a tyrosine phosphatase activity of tumor suppressor genes now that the PTEN gene and its regulation of the Akt pathway in prostate cancer prevention and treatment has an important role [1]. 3’-daidzein sulfonate (3’-daidzein sulfonate sodium, DSS) is th