7nitroindazole deep hypothermic circulatory arrest on cerebral protection in rats.docVIP

 PAGE \* MERGEFORMAT 14 7nitroindazole deep hypothermic circulatory arrest on cerebral protection in rats Of: Liuzun Tao, Chen Suocheng, Han Fu, Ming-governance [Abstract] Objective: To investigate the neuronal nitric oxide synthase (nNOS during deep hypothermic circulatory arrest (deep hypothermic circulatory arrest, DHCA brain injury in rats. METHODS: 154 healthy SD rats were randomly divided into 4 groups: sham group, DHCA group, 7-nitroindazole (7 NI group and the peanut oil group, divided into 3 groups after DHCA after 2,6,12 and 24 h 4 subgroups. sham operation group and 24 h subgroups The 16 rats, and the remaining sub-group of 10 rats, the ice when the embedded temperature dropped to 21 to establish bilateral common carotid artery occlusion model of DHCA. observe the specific nNOS inhibitor 7 NI on brain edema, brain tissue light microscopy, electron microscopy structure and content of MDA in brain tissue. Results: Compared with the DHCA group, 7 NI can reduce brain edema (P lt;0.05, improve brain cell shape, and can reduce the MDA content (P lt;0.05 . CONCLUSION: nNOS from brain injury in the DHCA cytotoxicity, 7 NI there is hope to extend time limit for DHCA security. [Keywords:] deep hypothermic circulatory arrest, nitric oxide, nitric oxide synthase, 7-nitroindazole [Abstract] Objective: To investigate the function of neuronal nitric oxide synthase (nNOS) in deep hypothermic circulatory arrest. Methods: One hundred and fifty four SD rats were randomly divided into 4 groups: fake operation group (n = 16), DHCA group (n = 46), 7 nitroindinazole (7 NI) group (n = 46), arachisoil group (n = 46). The last 3 groups were each divided into 4 subgroups according to the time phase after DHCA, which was 2 h ( n = 10), 6 h (n = 10), 12 h (n = 10), 24 h (n = 16). Imbedding rats by ice cubes to reduce the temperature and occlusion both common carotid arteries while the temperature reach 21 . The effect of nNOS inhibitor 7 NI on ischemia edema, struc