About 1 826 cases of prenatal diagnosis of chromosome.docVIP

 PAGE \* MERGEFORMAT 10 About 1 826 cases of prenatal diagnosis of chromosome Study: Stem Fangxiao Wu Zhi-Feng Zhao Jing Liang Shaoxia on Residents [Keywords: prenatal diagnosis of chromosome Fetal chromosomal abnormalities can lead to birth defects. Current conditions in China generally carried out in areas of prenatal screening for chromosomal abnormalities and prenatal diagnosis. As medical technology continues to evolve, more and more perfect method of prenatal diagnosis, in recent years got more and more applications. are prenatal chromosomal diagnosis in our hospital were retrospectively analyzed data. Subjects and methods 1. Object: August 2006 ~ August 2009 to our hospital for prenatal diagnosis prenatal diagnosis center for pregnant women 1 826 cases. Amniocentesis 713 cases, 15 to 23 weeks gestational age; cord blood puncture 1 113 cases of pregnancy 21 weeks to 36 weeks. Prenatal diagnosis of Down’s screening who meet high-risk accounts for 43.4% (792 / 1 826), agegt; = 35 years old accounted for 20.9% (381 / 1 826), B abnormalities accounting for over 14.7% (269 / 1 826), history of adverse pregnancy accounted for 11.8% (215 / 1 826), genetic family history, 3.6% (65 / 1, 826), other 5.7% (104 / 1 826). pregnant women aged 20 to 43 years (mean 29.6 + - 5.6) years old. 2. Amniotic fluid culture: The amniotic fluid samples collected from 20 ml-packing tips at the end of two 15 ml centrifuge tubes, each 10 ml, 1 600 r / min centrifugation 10 min, the supernatant discarded, leaving about 0.5 ml of amniotic fluid, the amniotic fluid by adding 5 ml complete medium (U.S. CHANG medium), inoculated in 25 ml culture bottle, set 37 ℃, 5.0% CO2 environment, training, 6 d were observed under inverted microscope after the growth of cell clones. If adherent more intensive growth of cells, cloning more, the suspension will be two bottles of culture into the other a new culture bottles, each bottle in the original by adding 5 ml fresh medium, the