A simple method for detection of apoptosis.docVIP

 PAGE \* MERGEFORMAT 9 A simple method for detection of apoptosis [Abstract] Objective of artemisinin-induced apoptosis in human glioma U251 cells, for example, describes a simple method for detection of apoptosis. Methods treated with artemisinin, the suspension of cultured human glioma U251 cells with liquid nitrogen freezing and thawing, then add 3mmol / L of PI 30L, at room temperature for 5min, the use of flow cytometry in ploidy characteristics of apoptosis rate calculated. Results 1 10-4, 1 10-5 and 1 10-6mol / L dose, artemisinin-induced apoptosis in human glioma U251 cells were 33.2%, 24.0% and 8.0%. Conclusion The results show that artemisinin significantly induced apoptosis of U251 glioma and show a clear dose-dependent. This approach simplifies the process cells, avoiding many factors, is an easy, accurate and rapid apoptosis detection method. [Keywords:] artemisinin; apoptosis; flow cytometry; apoptosis detection ABSTRACT: Objective To introduce a simple and easy method for cell apoptosis analysis as an example with artemisinin to induce apoptosis of human U251 cells. Methods Human U251 cells, cultured in suspension and treated with artemisinin, were freeze thawed. 30L of 3mmol / L PI was then added and mixed. After being placed under room temperature for 5min, the cellular ploidy was analyzed by flow cytometry, and the ratio of cell apoptosis was calculated. Results The ratio of human U251 cell apoptosis induced by artemisinin at a dose of 1 10-4, 1 10-5 and 1 10-6mol / L was 33.2%, 24.0% and 8.0%, respectively. Conclusion The results showed that artemisinin significantly induced the apoptosis of human U251 cells in a dose dependent manner. Using this method to analyze cell apoptosis can reduce the procedure of cell treatment and avoid many influential factors. Therefore, it can be regarded as a simple, accurate and rapid method for detecting cell apoptosis. KEY WORDS: artemisinin; cell apoptosis; flow cytometry; apoptosis detect