About Hedysari flavonoids on human leukemia cells induced differentiation.docVIP

 PAGE \* MERGEFORMAT 9 About Hedysari flavonoids on human leukemia cells induced differentiation [Abstract] Objective Hedysari flavonoids on human promyelocytic leukemia HL-60 cell differentiation and its molecular mechanism. Methods HL-60 cells were treated Hedysari flavonoids, NBT reducing ability to detect cell changes, with cell cycle by flow cytometry and CD11b, C-fos expression. Results of total flavonoids Hedysari role in HL-60 cells, NBT reduction capacity enhancement, CD11b expression increased, cells were arrested in G0/G1 and G2 / M phase, S phase cells were reduced accordingly; C-fos gene expression increased. Conclusion Hedysari flavonoids can induce HL-60 cell differentiation and maturation, which may be with the increased C-fos gene expression. [Keywords:] Red Qi, flavonoid compounds, HL-60 cells, C-fos gene Qi is more than the red sequence legume Hedysarum (Hedysarum polybotrys Hand .- Mazz) dry root, astragalus in the top grade, with qi solid form, diuretic detoxification, the abscess, sore convergence myogenic and other effects. Flavonoids are a class of low molecular weight natural plant components, widely found in vegetables, fruits and medicinal plants, is more concerned about people of a class of compounds. such components of leukemia cell growth, differentiation, apoptosis and other aspects of its mechanism has also become the current hot spots. experiment Hedysari the total flavonoids induced differentiation of leukemia cells were studied to explore its mechanism of action of flavonoids as red Qi provide experimental basis for the development and utilization. 1 Materials Hong Qi Gansu Longxi produced genuine medicine, the hospital medicine identification of Department Professor Zhang Xiling identified as authentic, the total flavonoids from this laboratory isolated and purified, the content through the determination was 88.3%. RPMI1640 for American Sigma. Newborn calf serum were purchased from Evergreen Biological Engine