About piperine in C57BL - 6 mice of gallstone formation.docVIP

 PAGE \* MERGEFORMAT 9 About piperine in C57BL / 6 mice of gallstone formation Of: Wang Haimei, Batuwula, Wu Jingtao, Liu Shaohui, An Guoshun, Niju Hua, Li Yueting, Zhu Xueguang [Abstract] Objective To observe piperine on experimental C57BL / 6 mice were gallstone formation. Methods C57BL / 6 mice 30, 10 in the control group, normal diet, the stone group, 10 rats fed the cholesterol diet containing 1% , treatment group, 10 rats fed with food containing 1% cholesterol diet and piperine (30 mg / kg body weight) .4 weeks were used reverse transcription - polymerase chain reaction (RT-PCR) and the chemical used to detect the level of liver Scp2 mRNA and changes in biliary lipid and cholesterol saturation index (CSI) by Carey scales. Results stones appear gallstone group 9 and 10 appear only cholesterol crystals, piperine group and the control group had no stones and crystals form, with the stone group, piperine Scp2 mRNA levels in liver and gallbladder bile CSI was significantly reduced. Conclusions of piperine in the apparent inhibition of C57BL / 6 mice were gallstone formation, while significantly lower levels of liver and bile Scp2 mRNA CSI, whether this is the prevention of piperine stone formation need further study. Keywords:] piperine, gallstones, sterol carrier protein 2, the cholesterol saturation index, mice Abstract: Objective To observe the effect of piperine (PA) on experimental gallstone formation in the gallbladder of C57BL / 6 mice. Methods 3 dietary groups of C57BL / 6 with 10 mice each group were allocated as control (normal mice chow), lithogenic ( 1% cholesterol diet) and PA (1% cholesterol diet + PA 30 mg / kg body weight) group respectively for 4 weeks. The expression of Scp2 gene in liver tissue was measured by RT-PCR and the bile lipid contents was measured chemically, the cholesterol saturation index (CSI) was calculated by Carey’s method. Results Cholesterol crystals and stones were found in 10/10 and 9 / 10 gallbladders respe