Acanthopanax tea on blood glucose in diabetic mice and anti-oxidative capacity of the.docVIP
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Acanthopanax tea on blood glucose in diabetic mice and anti-oxidative capacity of the
[Keywords:] Acanthopanax tea; diabetes; mouse; blood glucose; antioxidant capacity
Acanthopanax is a deciduous plant, with the ginseng belong to Araliaceae, mainly in China’s Northeast and North China. In recent years, studies at home and abroad are giving Acanthopanax.
1 Materials and methods
1.1 Drugs and equipment
Acanthopanax (Jilin Province, Li biogenic Biological Products Co., Ltd.), Alloxan (Shanghai Chemical Reagent Company), blood glucose kit (Beijing BIOSINO biotechnology company), superoxide dismutase (SOD) kit, Gu glutathione peroxidase (GSH Px) kit, total antioxidant capacity (T ADC) kits and malondialdehyde (MDA) kit (Nanjing Jiancheng Bio-Engineering Research Institute), 722 type sub-photometer (Shanghai Third Analysis Instrument Factory), E12140-type electronic balance (United States).
1.2 Animal Model and Grouping
Healthy male Kunming mice 60 (Jilin University School of Medicine Department of Laboratory Animal Center), weight (20 ± 2) g. Basic materials to adapt to 1 week later, 10 mice were randomly taken as normal control group, the remaining copy the diabetes model mice. Fasting mice 18 h, the aqueous solution by alloxan 200 mg / kg was injected under the abdominal cavity of a second. 5 d after injection of orbital blood, glucose oxidase determination of fasting blood glucose, and its value is greater than 10.00 mmol / L were as tfkpsajd successful model [1], modeling the success of randomly selected 20 mice were divided into experimental control group and the barbed 5 plus tea and experimental groups of 10 rats. Take Acanthopanax (6 g) by adding hot water 100 mL, filtered after 0.5 h soaking. Acanthopanax tea each group of mice fed tea per day 1.0 mL, normal control group and experimental control group fed with the corresponding volume of distilled water, the cycle for 4 weeks.
1.3 Determination of indicators
End
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