About Salvia on the murine melanoma cell line B16-BL6 metastatic ability of invasion and.docVIP

 PAGE \* MERGEFORMAT 13 About Salvia on the murine melanoma cell line B16-BL6 metastatic ability of invasion and Of: Xu Ya, Gu Qin, Xia Weijun Cheng Logan [Abstract] Objective To study traditional Chinese medicine Danshen on highly metastatic mouse melanoma cell line B16-BL6 growth, adhesion, invasion, movement, transfer ability, and to explore its mechanism. Methods of using MTT method Salvia B16-BL6 cell proliferation effect, the use of cell adhesion experiment, reconstituted basement membrane invasion assay, chemotaxis experiments and exercise capacity spontaneous metastasis of mouse melanoma models were miltiorrhiza on B16-BL6 cell growth, adhesion, invasion , exercise, and metastasis. The results Danshen Extract on B16-BL6 cells with two-way adjustment, can significantly promote the B16-BL6 cells and basement membrane components fibronectin adhesion, significantly inhibited B16-BL6 cell invasion of basement chemotactic membrane capacity and athletic ability, can significantly reduce the volume of lung metastatic nodules, but no obvious effect on the number of nodules. Conclusion Salvia extract can significantly reduce the highly metastatic mouse melanoma cell line B16-BL6 lung metastasis may be related to inhibition of B16-BL6 cell invasion of basement membrane and reduce the B16-BL6 exercise capacity. [Keywords:] Salvia, melanoma cell line B16-BL6 Abstract: Objective To study the effect of Radix Salviae Miltiorrhizae (RSM), a kind of Chinese Traditional Medicine which can dissolve stasis by activating blood circulation, on proliferation, invasion, adhesion, migration and metastasis of B16-BL6 metastatic mouse melanoma cells and discuss its functional mechanism. Methods The proliferation, adhesion, invasion and migration capacity of B16-BL6 metastatic cells was evaluated by MTT assay, adhesion assay and reconstituted basement membrane invasion and migration assay in vitro respectively. Mouse spontaneous melanoma model was used to study the ef