Acetylcholinesterase inhibitory activity of endophytic fungi Huperzia serrata Screening.docVIP

 PAGE \* MERGEFORMAT 10 Acetylcholinesterase inhibitory activity of endophytic fungi Huperzia serrata Screening Author: Cheng Xi Mr Philip Chan, Zhu Da-Yuan Guo Shangbin [Abstract] Objective To screen the acetylcholinesterase inhibitory activity of Huperzia serrata strains of endophytic fungi. Methods chromogenic DTNB method, optimizing the conditions screened; right Huperzia serrata of the 29 strains of endophytic fungi fermentation liquid alcohol extract for the enzyme inhibitory activity of the initial screening. Results xg, g8, g5, xgc2, xy7 on acetylcholinesterase inhibition with high rates, their IC50 were 285.62,293.53, 434.17, 668.82,856.21 μ g / ml. Conclusion Huperzia serrata endophyte xg, g8, g5 fermentation alcohol extract could more significantly inhibit acetylcholinesterase, AChE inhibition in search of a medicinal resources, has opened up a new way. [Keywords:] Acetylcholinesterase Huperzia serrata endophytic fungi Alzheimer’s disease (Alzheimer disease, AD) is a serious hazard to human health diseases. It is one of the pathogenesis of nerve impulse conduction in the course of a lack of acetylcholine. Acetyl cholinesterase (acetylcholinesterase, AChE) break down acetylcholine, so that treatment strategy for AD, is to use an AChE inhibitor to reduce the consumption of acetylcholine, thereby enhancing cholinergic neuron excitability, the effect of easing the symptoms [1]. Medicinal Plants of China’s pharmaceutical workers from Huperzia serrata Huperzia serrata (Thunb.trev) extracted to be effective, reversible AChE inhibitor - Huperzine A (HupA) [2]. As Huperzia serrata natural resources depleted, limiting the development of Huperzine A use. Scholars at home and abroad mainly through chemical synthesis, plant tissue culture to solve the problem of lack of natural resources [3]. This paper attempts from Huperzia serrata looking for endophytic fungi with AChE inhibitory activity of the strains, in order to improve Huperzia serrata to find a poss