Act modified tissue explant cultured rat airway smooth muscle cells.docVIP

 PAGE \* MERGEFORMAT 10 Act modified tissue explant cultured rat airway smooth muscle cells [Abstract] Objective: To improve the rat airway smooth muscle cells (airway smooth muscle cells, ASMCS) of the training methods, to understand the growth characteristics, the study provides in vitro studies of airway disease model. Methods: The animals were anesthetized and the rapid separation bronchial tree, removal of internal and external membrane and used 0.2% I collagenase digestion, using modified tissue explant culture method ASMCS, Morphological and anti-SM @-action were identified by immunocytochemistry smooth muscle cells. Results: The modified tissue explant culture method 2 ~ 6 d, cells begin to grow from the surrounding tissue, 5 ~ 8 d in the tissue near the “Valley - peak” structure, 9 ~ 12 d can be integrated. passaged cells by SM @-action immunohistochemical SP staining method rate of 95% or more. Conclusion: The modified tissue explant method of airway smooth muscle cells cultured with simple, easy, high yield, high purity of the advantages for the study of airway disease in vitro studies provide a good model. [Keywords:] airway; smooth muscle; cell culture; tissue attached method; rat Airway smooth muscle cells (airway smooth muscle cells, ASMCS) is an important structural airway cells in one of the important physiological role. ASMCS exception is the important pathological feature of respiratory diseases, the proliferation of airway remodeling in hypertrophy is Key [1]. Hirst et al [2] that the study should be smooth muscle cells as targets of asthma. ASMCS is to study the in vitro behavior of cell biology, disease pathogenesis and control means basis. In this study, modified tissue attached method train ASMCS, reported as follows. 1 Materials and methods 1.1 Animals SPF SD male rats were purchased from Shanghai Experimental Animal Center, weighing 250 ~ 350 g. raised in the Wenzhou Medical College, Laboratory of Experimental Animal