Adenovirus-mediated p53 gene on human hepatocellular carcinoma cell inhibition of experimental study.docVIP

 PAGE \* MERGEFORMAT 7 Adenovirus-mediated p53 gene on human hepatocellular carcinoma cell inhibition of experimental study Author: Tako Liu Ji-Liang Dong Song Chen Wei-hong Tan Shu-yu [Abstract] Objective: To study adenovirus-mediated wild-type p53 gene on human hepatoma cell line HepG2 in vivo. Methods: The MTT colorimetric assay of recombinant human p53 adenovirus (Ad-p53) in different infection titers and different infection time on HepG2 human liver cancer cell growth inhibition, using flow cytometry after Ad-p53 role in HepG2 the apoptosis rate; using intratumoral injection of Ad-p53 was observed in nude mice transplanted tumor in vivo. Results: Ad-p53 on HepG2 cells have significant inhibitory effect, effect-target ratio (MOI) the higher the role of the longer stronger inhibition; as rAd-p53 in the 125 MOI efficiency target ratio, the cell growth has basically reached the full inhibition; p53 transduction of HepG2 cells significantly increased apoptosis rate; intratumoral injection of Ad-p53, the tumor-bearing nude mice tumor growth was inhibited. Conclusion: Ad-p53 in human hepatoma HepG2 cells have significant inhibitory effect, its inhibitory effect with time, dose-dependent, Ad-p53 transduced wild-type p53 gene may be an effective means of gene therapy for hepatocellular carcinoma. [Keywords:] liver cancer; adenovirus; p53; gene therapy 〔 Abstract〕 Objective To explore the potential use of adenovirus mediated p53 gene (Ad-p53) therapy for hepatocellular carcinoma. Methods A human hepatocellular carcinoma cell line HepG2 was used. Recombinant adenovirus carrying wild-type p53 gene was transfected into HepG2 cells in vitro and injected into tumor nodules in vivo. The growth of HepG2 cells in vitro and established hepatocellular carcinoma nodules in nude mice was examined. Cell apoptosis was analysed by Annexin-V/PI labeling flow cytometry method. Results Cell growth was greatly suppressed at ≥ 125MOI . p53 transfection can increase HepG2 cells ap