Advances in detection methods of cytokinin.docVIP

 PAGE \* MERGEFORMAT 30 Advances in detection methods of cytokinin [Abstract] cytokinin is an important class of plant growth regulating hormones, these hormones on the quantitative detection of micro plants to reveal their signal transduction mechanism to play its role in regulating the growth of crops is important. This reviews recent studies of cytokinin progress of various analytical methods, including immunoassay, gas chromatography, high performance liquid chromatography, capillary electrophoresis and electrochemical methods, introduced before the actual treatment methods commonly used in the sample, and discussed the direction of future research. [Keywords:] cytokinin, immunoassay, chromatography, capillary electrophoresis, sample preparation, review 1 Introduction Cytokinin (cytokinins, CTKs) is an important class of plant growth regulating hormone, in plants in a wide range of biological processes. CTKs can promote the formation of plant roots, and root damage repair [1]. CTKs also affected the chloroplast manufacturing and micro-structure [2], affecting photosynthesis and resistance to pathogens [3]. At present, CTKs has been confirmed in more than 40 [4], all natural CTKs are adenine derivatives. Table 1 CTKs a typical structure, name and abbreviation. in accordance with its six N atoms (N6) of substituents (R1) of the different common CTKs can be divided into three categories: isoprene type, isoprene-derived and aromatic [4]. Also, according to the different R2 and can be divided into free base type, nucleoside type glucosidase type and nucleotide type. In addition there are three N atoms or N 7 atom on the glucose-substituted CTKs [5]. CTKs mainly produced in plants in the RNA metabolic process [6], biologists have been able to regulate the expression of genes to control the content of endogenous CTKs [7], but further determine the role of plant growth CTKs need accurate within Quantitative analysis of endogenous CTKs. In addition, i