Allogeneic immune response against ITAC and its receptor expression induced in vitro.docVIP

 PAGE \* MERGEFORMAT 12 Allogeneic immune response against ITAC and its receptor expression induced in vitro Of: Advanced Song Yanfang Zhu, Wei Xu group, Zhangqiu Yu, Su Donghui [Abstract] Objective To investigate the allogeneic immune response against interferon-inducible T-cell chemotactic agent @ subfamily (I TAC and its receptor CXCR3 expression. Methods of allogeneic peripheral blood mononuclear cells (PBMC after mixed culture , the supernatant effect on ECV304 cell line, RT PCR detection of the I TAC mRNA expression in ECV304, ELISA detection of IFN in culture supernatants and TNF @ concentration of PBMC detected by flow cytometry the surface expression of CXCR3. The results with individual PBMC culture group, mixed group of PBMC culture supernatant cytokine interferon (IFN , tumor necrosis factor-@ (TNF @ expression was significantly increased, and can promote the ECV304 cell I TAC mRNA expression, after the mixed culture PBMC surface expression of CXCR3 was also found significantly increased. Conclusion allogeneic immune response may be by cytokines, chemokines and I TAC and its receptor in T cells the expression of CXCR3 in order to increase positive feedback in the form allograft rejection reaction. [Keywords:] Transplantation, Homologous, chemokines, receptors, chemokines, endothelial cells, endothelium, blood vessels, umbilical vein In organ transplantation, recipient T cells were collected and entered through the endothelial cell layer is the host anti-graft acute and chronic graft rejection are the most important conditions. Interferon-inducible T-cell chemotactic agent @ subfamily (IFN inducible T cell alpha chemoattractant, I TAC is a recently discovered chemokine, also known as CXCL11, its receptor CXCR3, expressed in the Th1 cell surface. I TAC Th1 cells have been shown to have a strong raising effect, thus causing transplant rejection is considered to be one of the key factors [13]. has been shown, IFN can induce astrocytes, br