Amantadine and oseltamivir against H5N1 in vitro the role of avian influenza virus subtype.docVIP

 PAGE \* MERGEFORMAT 19 Amantadine and oseltamivir against H5N1 in vitro the role of avian influenza virus subtype Of: Liu Lihua Zhu Xiang Zhang Yufeng Zhang Wenjing Yun Lin Chao Ying standard double Liao Ming Xian Zhang Kou-xing Xin Chaoan [Abstract] [Objective] To observe the in vitro single-use amantadine, oseltamivir and the combination of two drugs on the two H5N1 subtype avian influenza virus isolates (AIV127, AIV174) inhibition, to understand the different isolates in Guangdong H5N1 strain of the differences between the drug sensitivity for clinical application of antiviral drugs provide the scientific basis. [Method] established in vitro model of H5N1 infection, according to drugs and viruses, cells of different role, through three kinds of delivery methods, the use of cell lesions (CPE), infected half the amount of tissue culture (TCID50) assessment of antiviral drugs against H5N1 in vitro. [results] of three delivery methods combined amantadine, oseltamivir, amantadine alone than in vitro inhibition AIV127 and AIV174 obvious effect (P lt;0.01); and only mode of administration in the treatment of the two combined than single effect of oseltamivir was significantly (P lt;0.01). Three mode of administration of amantadine, oseltamivir and combined on the same subtype H5N1 AIV127 than sensitive strains than AIV174 (P lt;0.01). [Conclusion] The antiviral drugs for avian influenza virus strains of different sensitivities between the different, should be based on different strains to give the corresponding antiviral therapy. [Keywords:] drugs; avian influenza virus; H5N1 subtype; anti-viral activity Abstract: [Objective] To observe inhibition of Amantadine, Oseltamivir and double combination on H5N1 in vitro and further investigate antiviral activity of drugs against H5N1 isolated locally. [Methods] Cytopathic effect (CPE), hemagglutinin (HA) titers and TCID50 of virus were applied to observe the antiviral activity of H5N1 (AIV 174 and AIV 127) wi