Angiopoietin.docVIP

 PAGE \* MERGEFORMAT 21 Angiopoietin Keywords: angiogenin; tumor; angiogenesis; angiogenesis [Keywords:] Angiopoietin; tumor; angiogenesis; angiogenesis As early as 1971, American scholar Folkman [1] proposed an tumor growth and metastasis depend on angiogenesis, in the ensuing decades, an increasing number of studies confirm this point. Angiopoietin (angiopoietin, Ang) is the first to be established from human tumor tissue with angiogenesis effect of cytokines [2]. Now known to the family, including Ang1, 2,3,4 four members, Ang1, 2 and angiogenesis most closely, and about the family members in tumor angiogenesis research is increasing. Ang1 and Ang2 are now on the role of tumor angiogenesis are reviewed. 1 Ang1 and Ang2 the structure and biological function of 1.1 Ang1 and Ang2 the structure of Ang1 gene in 1996 by Davis et al [3] for the first time cloned. Human Ang1 gene located in long arm of chromosome No. 8 on the (8q22.3 ~ q23), its gene open reading frame of 1497bp, encoding 498 amino acids. Ang1 is a glycoprotein, the molecular weight of about 75,000. Ang2 gene was identified by Maisonpierre et al [4] in 1997 from the human and mouse cDNA library cloned in the first. Human Ang2 gene location on chromosome No. 8 of the short arm of (8q23.1), its gene open reading frame of 1491bp, encoding 496 amino acids. Ang1, Ang2 protein structure is basically the same, have the signal peptide, N terminal coiled-coil domain (coiledcoil domain, CC) and the C terminal fibrinogen domain classes (fibrinogenlike domain, FL). Its main structural features are: (1) Ang1, Ang2 N-terminal signal peptide by 10 and 20 hydrophobic amino acids, and angiopoietin-related secreted outside the cell. (2) The coiled-coil domain, respectively 180 and 200 or so amino acid composition, amino acid sequence of the fault bend folding to form a coiled-coil quaternary structure, this structure may be related to angiopoietin, and other proteins related to the format