Application of siRNA technology reduced survivin gene expression in SGC7901 gastric cancer cells increased the sensitivity to cisplatin.docVIP

 PAGE \* MERGEFORMAT 13 Application of siRNA technology reduced survivin gene expression in SGC7901 gastric cancer cells increased the sensitivity to cisplatin [Abstract] Objective use of small interfering RNA (siRNA) technology-specific down survivin gene expression, detection of SGC7901 gastric cancer cells transfected changes in sensitivity to cisplatin. Methods were divided into control group (control group), alone Soon Platinum group (CDDP group), liposome (Lip group), single group with survivin siRNA (siRNA group), transfected with survivin siRNA (transfection group) and survivin siRNA transfection combined with cisplatin group (joint action group) . synthetic survivin siRNA, siRNA transfected by Lip parcel into gastric cancer cells SGC7901, observed under a fluorescence microscope transfection. MTT assay of the inhibition of cell proliferation, Western blot and immunocytochemistry detected the expression of survivin protein, Hoechst staining morphological changes of apoptosis. Results Hoechst staining under fluorescence microscope control group, Lip group and siRNA group showed normal nuclei blue, while the CDDP group, transfection group and enhance joint action group nuclei, nuclear chromatin condensation, nuclear fragmentation, showing apoptotic changes; combined effects of cell proliferation inhibition rate (63.93 + -4.22)% was significantly higher than other groups (P lt;0.05); transfected cells and survivin protein expression in the joint action group was significantly lower than other groups (P lt;0.05). Conclusion The application of siRNA technology in gastric cancer cells SGC7901 down survivin gene expression, to increase their drug sensitivity to cisplatin. [Keywords:] survivin; siRNA; gastric cancer; cisplatin Abstract: Objective To detect the variations in cisplatin sensitivity of human gastric carcinoma cell line SGC7901 before and after transfection to specifically downregulate the expression of surviving gene with small interfering