Artemisia annua leaf callus induction and plant regeneration.docVIP

 PAGE \* MERGEFORMAT 13 Artemisia annua leaf callus induction and plant regeneration Author: Wu Xiaoli, Liu Fei, Li Longyun, Zhong-Yue 【Abstract】 Objective To explore these young leaves of Artemisia annua plant regeneration method. Approach to these young leaves of Artemisia annua explants inoculated on MS supplemented with different hormone combinations of medium, for callus induction and plant regeneration. Results The 6  BA0.1 mg / L 2,4-D0 .3 mg / L induced callus containing 6  BA0.1 mg / L IAA0.3 mg / L of the medium division containing IAA0 .1 mg / L NAA0.3 mg / L medium roots. Conclusion The above method can be in a relatively short period to be regenerated from these young leaves of Artemisia annua plants. Keywords: Artemisia annua; plant hormones; callus; regeneration Abstract: ObjectiveTo explore the way of regeneration of plants from leaves of Artemisia annua L.. MethodsTo plant the leaves of Artemisia annua L. on mediums plus different plant hormones. ResultsThe suitable callus-inducing medium was MS 6  BA1.0 mg / L 2,4  D0.3 mg / L. The suitable regenerating medium was MS 6  BA0.1 mg / L IAA0.3 mg / L. The suitable root  inducing medium was MS IAA0.1 mg / L NAA0.3 mg / L. ConclusionYoung plants can regenerate from leaves in a short span by the method above. Keywords:: Artemisia annua L; Plant hormones; Callus; Regeneration Artemisia annua (Artemisia annua) L. , Also known as Artemisia annua, a medicinal herb annual Asteraceae. Extract artemisinin malaria and cerebral malaria chloroquine antimalarial properties of a highly efficient and low toxicity, WHO has identified compound artemisinin-based medicines for the most safe and effective antimalarial drugs. Now the world has 51 countries and regions in the WHO recommendation be designated as anti-malaria medication. This makes the global demand for artemisinin-based raw materials soared, beginning in 2004 have emerged from the raw materials to finish