Artesunate on human liver cancer HEPG-2 cells in vitro.docVIP

 PAGE \* MERGEFORMAT 5 Artesunate on human liver cancer HEPG-2 cells in vitro [Abstract] Objective To study the artesunate (Artesunate, Art) inhibition of tumor cell proliferation. Methods MTT Determination of Art, and iron combined with, as well as 5-FU combined with human hepatocellular carcinoma cell line HEPG-2 inhibition of proliferation. Results Art can inhibit the growth of HEPG-2 cells and showed significant time - dose effect. 24,48,72 h the IC50 values were 103.1,75.3,65.7 μ g / m. When the Art (10μ g/ml) with 5 - fluorouracil (5-Fu) (5μ g/ml) combination has shown significant synergy. Conclusion Art on human hepatocellular carcinoma cell line HEPG-2 cells showed significant inhibitory effects, it is possible to develop support for clinical anti-cancer treatment drug. [Keywords:] Artesunate HEPG-2 Abstract: ObjectiveTo study the effect of artesunate (Art) on HEPG-2 cells in vitro.MethodsCell culture, MTT were performed to observe the proliferation of Art, combining with iron ,5-FU. ResultsArt (50 ~ 200μ g/ml) could inhibit HEPG-2 cell proliferation in a time-dose dependent manner and IC50 were 103.1,75.3,65.7 μ g / ml in 24h, 48h, 72h respectively. When Art (10μ g/ml) was used combining with 5-Fu (2.5μ g/ml ), there was obvious synergies.ConclusionArt could significantly inhibit the proliferation of HEPG-2. Keywords:: Art; HEPG-2 Artesunate (artesunate, Art) is extracted from natural plant Artemisia annua in a peroxide-containing groups of sesquiterpene lactones, its structure has a certain novelty, mainly used for clinical treatment of malaria . The current study found that its also has anti-tumor effect [1,2]. This study of the Art on cultured human hepatoma cell line HEPG-2 cell proliferation. Are reported below. 1 Equipment 1.1 Instrument Automatic CO2 incubator box (USA Thermo Formo), inverted microscope (Nissan), Lei Bo MK3 microplate reader. 1.2 Reagents RPMI-1640 culture medium (Gibco U.S. companies); fetal calf serum (Hangzhou Sij