As2O3 on human lymphoma CA46 cell proliferation viability and cell cycle and its mechanism.docVIP

 PAGE \* MERGEFORMAT 14 As2O3 on human lymphoma CA46 cell proliferation viability and cell cycle and its mechanism [Abstract] Objective To study the arsenic trioxide (As2O3) on the proliferation of malignant lymphoma CA46 cells, and cell cycle activity and its mechanism. Methods sulfonyl rhodamine B (SRB) were observed at different concentrations (0.625,1.25,2.5,5.0, 10.0,20.0 mol / L) As2O3 treatment 24,48,72,96 h, respectively, after the CA46 cell line growth curve, and calculate the concentration of As2O3 treatment of the CA46 cell line after 72 h of cell growth inhibition rate; effects of different concentrations (0.5, 1.0,2.0 mol / L) As2O3 72 h, CA46 cell lines after cloning efficiency; flow cytometry DNA ploidy analysis of different concentrations (0.5,1.0 and 2.0 mol / L) As2O3 72 h, cells on the CA46 cell cycle; RT PCR assay with different concentrations (0.5,1.0 and 2.0 mol / L) As2O3 role in CA46 cells after 72 h p16 gene mRNA. while located in the control group for comparison. Results (1) and untreated group, the concentration of As2O3 could inhibit the growth of CA46 cells, and the G0 ~ G1 phase cells increased in a dose dependent manner. (2) The untreated cells showed a weak p16 gene expression, different concentrations of As2O3, 72 h, untreated group, different concentrations (0.5,1.0 and 2.0 mol / L) As2O3 treatment group positive for p16 gene expression and actin band intensity ratio values were (0.11 + -0.11), (0.33 + -0.10), (0.57 + -0.11) and (0.67 + -0.09), the p16 gene mRNA As2O3 treatment group was significantly higher than untreated group, the difference was statistically significant (P lt;0.01). Conclusions As2O3 could be cell cycle arrest at G0 ~ G1 period, inhibition of tumor cell growth, which might be induced by p16 gene expression. [Keywords:] Arsenic; lymphoma; cell proliferation; tumor cells, cultured; cell cycle; gene, p16 Arsenic trioxide (As2O3) is a recent discovered and used successfully in the domestic first acute