Asiatic acid inhibited HSCT6 cell expression of typecollagen protein.docVIP

 PAGE \* MERGEFORMAT 2 Asiatic acid inhibited HSCT6 cell expression of typecollagen protein [Abstract] Objective: To study exogenous TGF  β in hepatic stellate cells (HSC  T6) of activation, as well as Asiatic acid inhibited HSC  T6 cell type Ⅰ collagen synthesis pharmacological effects. Methods: LDH, and MTT Experimental Determination of asiatic acid on HSC  T6 cellular cytotoxicity and cell proliferation. Detection of HSC  T6 cells at different times by different doses and TGF  β -stimulated expression of type Ⅰ collagen on the optimum dose and time. with different dose of snow acid acting on TGF  β -activated HSC  T6 cells, extracted total cellular protein was detected by Western Blot snow acid on TGF  β -activated HSC  T6 cells, type Ⅰ collagen protein expression. Results: TGF  β cells to stimulate HSC  T6 type Ⅰ collagen on the optimum dose of 1 ng / mL, the best time to 24 h. 10 ~ 30 μ mol / L of snow acid can inhibit the TGF  β -induced collagen Ⅰ HSC cells protein expression. Conclusion: Exogenous TGF  β can activate HSC  T6 cells Collagen Ⅰ ; asiatic acid inhibited HSC  T6 cells in type Ⅰ collagen protein expression. [Keywords:] Asiatic acid; Ⅰ collagen; HSC  T6 cells; transforming growth factor  β 0 Introduction Centella asiatica has a traditional Chinese medicine to promote wound healing and inhibiting scar formation and efficacy [1-2]. Recent studies have shown that Centella asiatica can inhibit scar tissue-derived fibroblast proliferation and fiber protein synthesis [2]. Liver Star dendritic cells in hepatic fibrosis plays an important role. In this study, the role of snow-acid large T antigen in SV40 virus transformation of hepatic stellate cells (HSC  T6), study of asiatic acid on HSC  T6 cells collagen synthesis. 1 Materials and methods 1.1 Materials Asiatic acid (SIGMA, Cat: 546712), yellow-brown powder, odorless, bitter taste. Molecular formula C48H78O5, melting point 325 ℃ ~ 330 ℃ , i