Astragalus of high glucose and lipid environment endothelial cells and the interaction of mesangial cells.docVIP

 PAGE \* MERGEFORMAT 10 Astragalus of high glucose and lipid environment endothelial cells and the interaction of mesangial cells Of: Zhou Suxian, Zhao Jin Jin, Lei Min Xiang [Abstract] Objective To study the astragalus on the high glucose and lipid environment of endothelial cells and interaction of mesangial cells. Methods Endothelial cells were cultured mesangial cells and mesangial cells cultured alone, were divided into control group, mannitol group, high-sugar high-fat group, RA group, after 24 h culture, ELISA used to detect the supernatant of collagen type Ⅳ (Col Ⅳ), fibronectin (Fn) levels. results in high glucose and lipid environment of culture and single cell culture supernatant Col Ⅳ, Fn content was increased (all P lt;0.05), total supernatant Col Ⅳ, Fn higher than that of single culture (all P lt;0.05), RA group Col Ⅳ, Fn content high glucose and high fat group decreased (P lt;0.05). Conclusions high glucose and lipid environment, mesangial cells and endothelial cells in the abnormal interaction, this effect can promote Col Ⅳ, Fn generated. astragalus by inhibiting high glucose high-fat conditions, endothelial cells and the interaction between mesangial cells and reduce the Col Ⅳ, Fn content of the kidneys play a protective role. [Key Words] high-sugar high-fat, diabetic nephropathy, endothelial cells, mesangial cells, Astragalus Abstract: Objective To study the effect of astragalus membranaceus on the interaction of mesangial cells and endothelial cells in the media of high glucose and lysophosphatidylcholine. Methods The model of intercellular interaction between endothelial cells and mesangial cells of diabetic nephropathy was established and divided into 4 groups : control, mannitol, high glucose and lysophosphatidylcholine, intervented with astragalus membranaceus. Endothelial cells and mesangial cells were co-cultured in DMEN with or without astragalus membranaceus in high glucose and lysophosphatidylcholine media up to 24 hours.