Astragalus polysaccharides on murine dendritic cell precursors in vivo induction of.docVIP

 PAGE \* MERGEFORMAT 13 Astragalus polysaccharides on murine dendritic cell precursors in vivo induction of OF: Juan slightly, Zang Guoqing, Yu Yongsheng [Abstract] Objective To study the APS (astragalus polysaccharide, APS) whether in vivo induction of mouse marrow-derived dendritic cells (dendritic cell, DC) differentiation and maturation, to explain the mechanism of pharmacological APS. Methods the control group and the APS 100,200 mg / kg dose group, intraperitoneal injection, in vivo induced marrow-derived DC precursors and immature DC, weighing spleen weight, ELISA detection of serum GM-CSF expression, inverted cell growth state microscope, electron microscope to detect cell morphology, flow cytometry CD11c, MHC-Ⅱ molecules and costimulatory molecules CD80, CD86 expression levels. 7 d after injection of the results of APS, APS 100,200 mg / kg group of mice spleen weight was significantly increased compared with control group (P lt;0.01), bone marrow cells in vitro and by rmGM-CSF, rmIL-4 after the induction culture, flow cytometry showed that: APS100, 200 mg / kg group of CD11c, MHC- higher levels of class Ⅱ molecule expression (P lt;0.05), but CD80, CD86 expression was no significant difference compared with the control group, serum GM-CSF expression, the result was no significant difference with the control group. Conclusion APS could induce marrow-derived DC precursors to differentiate into DC, and may have to promote the role of DC maturation, but this effect is not GM-CSF by stimulating the body’s secretion of cytokines play a role. [Keywords:] dendritic cells, APS; mice Abstract: Objective To investigate the effect of astragalus polysaccharide, a component of an aqueous extract of Astragalus Membranaceus roots, on differentiation and maturation of dendritic cells in vitro. Methods 30 BALB / c mice were randomly divided into three groups, normal control group, 100 , 200 mg / kg APS intraperitoneal injection groups. After one week, weight