Asiatic acid and chemotherapy combined with anti-tumor effect of the preliminary study.docVIP

 PAGE \* MERGEFORMAT 13 Asiatic acid and chemotherapy combined with anti-tumor effect of the preliminary study Of: Na Lin, Gao Jing, Fang Chun Qian, Zhang Haifeng, Yang Jian, Gu Yajun, Guo Wenjie [Abstract] Objective: To study the asiatic acid (asiatic acid, AA and the chemotherapy drug cyclophosphamide (CTX, 5-fluorouracil (5 Fu combined with the anti-tumor effect. Methods: in vivo and in vitro combination of methods to observe AA and 5 Fu or CTX combined with the proliferation of hepatoma cell line HepG2 hepatoma ascites and Heps tumor, solid tumor growth. by MTT assay 5 Fu combined with AA and anti-tumor effect. Results: 10,15,20 , 25 mol / L AA and 50,7.5,1 mol / L 5 Fu MS, has significant anti-tumor effect, AA does not reduce the 5 Fu on HepG2 cell proliferation. AA and CTX together for small Heps ascites tumor mice and found that AA (50,100 mg / kg and CTX (20 mg / kg combined with CTX alone, like with, can inhibit the proliferation of ascites, and extend the life of Heps mice ascites, AA, and CTX together for Heps solid tumor in mice, a significant inhibitory effect, and can resist the CTX-induced AA glutathione (GSH levels decrease, the liver may have some antioxidant protection. Conclusion: AA and combined with chemotherapy drugs can be reduced toxic side effects of chemotherapy drugs. [Keywords:] asiatic acid, 5-fluorouracil, cyclophosphamide, combination therapy, anti-tumor [Abstract] Objective: Study the anti tumor effects of asiatic acid (AAcombined with chemotherapeutics cyclophosphamide (CTX) or 5 fluorouracil (5 Fu). Methods: The cytotoxic effects of the combination of AA and 5 FU on tumor cell lines HepG2 were detected by MTT assay.Mice transplanted with Heps solid tumor and ascites carcinoma were used to study the combination anti tumor effect of AA (50,100 mg / kg) and CTX (20 mg / kg). Results: When AA (10, 15, 20, 25 mol / L) and 5 Fu (50, 7.5, 1 mol / L) was combinated to treat the cells, the growth of HepG2 cells were inhibite