Asiatic acid in diabetic rats and the mitochondria in the heart.docVIP

 PAGE \* MERGEFORMAT 18 Asiatic acid in diabetic rats and the mitochondria in the heart Of: the money side of spring, Gao Jing, Zhou Jun, Chen Yan, Wang Li, Wu Chenguang [Abstract] Objective: To investigate the asiatic acid (asiatic acid, AA) diabetes (diabetes mellitus, DM) rats and its possible mechanism of cardioprotection. Methods: 65 mg / kg streptozotocin (streptozocin, STZ) intraperitoneal injection of diabetic rats were divided into DM group, 10 mg / kg AA (DM + AA1) group and 40 mg / kg AA (DM + AA2) group. ig echocardiography 8 weeks after detection of cardiac function, detection of myocardial tissue and its mitochondrial superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and the ATP enzyme (ATPase) content or activity, mitochondrial membrane potential of myocardial (m), HE cardiac morphology were observed. pretreated with different concentrations of AA mitochondria, was detected on the hydroxyl radical ( OH) caused by mitochondrial injury. Results: DM group left ventricular ejection fraction and left ventricular shortening fraction was significantly increased, cardiac SR appears aggregation, interstitial edema and other pathological conditions; DM rats heart tissue SOD activity was significantly decreased, MDA content increased significantly; myocardial mitochondria m, ATPase, SOD, CAT activity was significantly decreased. AA to some extent inhibitory effects were observed. In addition, AA can prevent Fe2 + + VitC induced mitochondrial lipid peroxidation. Conclusion: AA on the improvement of DM rat heart damage may be related to mitochondria, inhibits mitochondrial lipid peroxidation and redox regulating the balance of the heart. [Keywords:] asiatic acid; diabetes; heart; mitochondria; redox [Abstract] Objective: To observe the protective effect of asiatic acid (AA) on hearts and relative mitochondria in streptozotocin (STZ) induced diabetic rats. Methods: Diabetes group induced by STZ (65 mg / kg) and therapeutic group fol