Astragalus toxicity of bupivacaine spinal nerve injury effects of p38 kinase.docVIP

 PAGE \* MERGEFORMAT 6 Astragalus toxicity of bupivacaine spinal nerve injury effects of p38 kinase Author: Wang Jianxin Cui Rui Qiu Tingting Yue [Keywords:] astragalus; bupivacaine; nerve toxicity; p38 kinase Obstetric patients in our use of spinal anesthesia for cesarean section anesthesia or analgesia in labor and so on, some of which patients had local anesthetic because of nerve toxicity caused by different degrees of neurological complications. Domestic Professor Chen Bingxue collected from 2003 to 2005, Guangdong Province, 3 3 B hospitals to initiate spinal - epidural anesthesia cesarean section 800 cases, of which 7 patients had permanent nerve damage (Bryan school. ‘Combined spinal and epidural anesthesia concurrent Nerve damage local anesthetic toxicity ‘.2006 anesthesia, Guangdong Province, Annual Meeting thesis). Astragalus has a clear free radicals, lipid peroxidation and resistance to protect the function of SOD activity and thus play a neuroprotective effect [1], while Astragalus game of the spinal nerve toxicity of local anesthetics have protective effects have not been reported. In this study, pregnant rats produced intraspinal injection model, by observing the toxicity of Astragalus on bupivacaine spinal nerve injury in dorsal root ganglion p38 kinase, and to explore game Astragalus spinal anesthetic neurotoxicity protection mechanism. 1 Materials 1.1 Animals Healthy female SD rats weighing 180 ~ 200 g, by the Experimental Animal Center of Guangdong Province. At room temperature for 18 ~ 28 ℃ , relative humidity 40% ~ 70% of the barrier system, feeding, does not control diet, estrus the female rats and male rats (5:1 ratio) with cage rearing, daily line of vaginal Tu films, microscope to see the sperm as the 1st day of pregnancy. Pregnancy 17, pregnant rats were selected for the study. 1.2 Drugs and reagents Astragalus (9 Hong Chengdu Diao Pharmaceutical), bupivacaine hydrochloride powder (sigma company, batch number 014,252,803), rabb